基于“多肽组-修饰组”比较分析鹿皮与鹿皮胶物质基础

Comparative analysis of chemical constituents of deer hide and deer hide gelatin by "peptidomics-modifications" methods

胶原蛋白为胶类动物药主要物质基础,鹿皮在高温煎煮熬制加工成鹿皮胶的过程中胶原蛋白的部分氨基酸位点会发生羟基化与脱酰胺修饰,本文基于"多肽组-修饰组"结合的研究思路系统比较了鹿皮与鹿皮胶的蛋白质肽类物质基础。采用纳升液相-串联质谱法分析鉴定鹿皮与鹿皮胶中蛋白质类、肽类成分组成,并进一步比较鹿皮与鹿皮胶的修饰数量与修饰位点。结果表明,鹿皮胶的羟基化修饰与脱酰胺修饰数量均显著多于鹿皮,提示在高温熬制加工过程中,鹿皮胶原蛋白的部分氨基酸位点发生了修饰,这些修饰与胶原蛋白的特定氨基酸结构单元、亲疏水性等因素有关,鹿皮胶原蛋白发生修饰的阶段与机制有待后续研究阐释。本文为鹿皮胶物质基础研究、加工过程及胶原蛋白修饰的相关性研究提供了重要理论依据,有利于开展鹿皮胶工艺优化、质量标准提升等研究。

Collagen is the main constituent of gelatinous Chinese medicine,with deer hide gelatin(Cervi Corii Colla,DHG)made from deer hide(DH)through a complex thermal and high-pressure processing procedure.During this procedure some amino acids in collagen undergo hydroxylation and deamidation.In the present study,comparative analysis of proteins and peptides in DH and DHG was carried out using"peptidomics-modifications"methods.Nano-LC-MS/MS was used to analyze proteins and peptides in DH and DHG,and the number and sites of modification were determined as well.The amount of hydroxylation and deamidation that occurred in DHG was significantly greater than that in DH,suggesting that under thermal and high-pressure processing these modifications occurred more frequently on certain amino acids in collagen,and might be correlated with hydrophobicity.The occurrence and mechanism of hydroxylation and deamidation in DH processing procedures should be explored in further research.The present study provides important evidence of the chemical constituents and the correlation of processing procedures with these modifications,and also suggests some investigative ideas for DHG processing optimization and improvement of quality standards.