基于网络药理学竹沥活性成分甲酸治疗糖尿病的分子生物学机制研究

Study on Molecular Biological Mechanism of FMT,an Active Ingredient of Bamboo,Based on Network Pharmacology in the Treatment of Diabetes Mellitu

目的运用网络药理学和生物信息学分析方法,研究竹沥活性成分甲酸(FMT)治疗糖尿病(DM)的分子生物学机制。方法利用TCMSP数据库筛选FMT的作用靶点,利用NCBI数据库获得DM相关靶点蛋白,对两者靶点进行交互处理,利用STRING数据库对靶点蛋白互作进行分析,使用Cytoscape构建蛋白互作网络,利用DAVID数据库和gProfiler数据库进行GO分析和KEGG信号通路富集分析。结果FMT作用靶点232个,DM相关靶点蛋白1958个,重叠靶点58个,其主要涉及酪氨酸代谢信号通路、花生四烯酸信号通路、苯丙氨酸代谢信号通路。结论FMT治疗DM具有多靶点、多途径的特点,为深入研究其作用机制提供了理论依据。

Objective To study the molecular biological mechanism of bamboo active ingredient FMT in the treatment of diabetes by using network pharmacology and bioinformatics analysis methods.Methods The target of FMT was screened by TCMSP database.The target protein of diabetes was obtained by NCBI database.The two targets were treated interactively.The interaction of target proteins was analyzed by STRING database.The protein interaction network was constructed by using Cytoscape.The DAVID database and the gProfiler database were used for GO analysis and KEGG signal path enrichment analysis.Results There were 232 targets for FMT,1958 for diabetes-related target proteins and 58 overlapping targets,mainly involved in tyrosine metabolic signaling pathway,arachidonic acid signaling pathway,and phenylalanine metabolic signaling pathway.Conclusion FMT treatment of diabetes has the characteristics of multiple targets and multiple pathways,which provides a theoretical basis for further study of its mechanism of action.