基于响应面法的耐盐菌最优化固定化条件研究

The Research for the Optimum Immobilization Conditions of Salt-tolerant Bacteria Based on Plackett-Burman Design

以海藻酸钠(SA)为包埋剂、CaCl2为交联剂对耐盐菌株进行固定化,并用于处理高盐废水。结果表明,单因素实验得到的优化条件为:SA和CaCl2的质量分数分别为2%和2%,进水COD为8 g/L,包埋剂pH为7,包埋量2.5 g/L,进水盐度3%,交联时间24 h。Plackett-Burman实验设计得到影响固定化菌株处理高盐废水的显著因素为包埋量、进水COD以及SA含量。Box-Behnken设计-响应面法运用二次方程对实验数据进行拟合确定优化条件为:包埋量2.33 g/L,进水COD为7.957 g/L,SA的质量分数1.94%,且三者之间交互影响明显,固定化耐盐菌颗粒处理废水效率可达72.45%。

Salt-tolerant strains were immobilized with sodium alginate as embedding agent and CaCl2 as crosslinking agent to treat the high salinity wastewater.The results showed that,the optimum conditions were obtained by single factor experiment as follow,mass fraction of sodium alginate(SA)and CaCl2 was 2%,influent COD was 8 g/L,pH of embedding medium was 7,embedding amount was 2.5 g/L,influent salinity was 3%,cross-linking time was 24 h.From the Plackett-Burman experimental design,it was found that the significant factors affecting the treatment of high salt wastewater by immobilized bacteria were embedding amount,influent COD and SA content.Box-Behnken design-response surface regression analysis was conducted based on quadratic equation to fit the experimental data,and the optimization conditions were determined as follows,embedding amount was 2.33 g/L,influent COD was 7.957 g/L and SA mass fraction was 1.94%.The interaction between the three factors was obvious and the wastewater treatment efficiency by immobilized salt-tolerant bacteria particles could reach 72.45%.