血清应答因子和肌球蛋白重链9在食管鳞癌组织中的表达及临床意义

Expression and clinical significance of serum response factor and myosin heavy chain 9 in esophageal squamous cell carcinoma

目的探讨血清应答因子(serum response factor,SRF)和肌球蛋白重链9(myosin heavy chain 9,MYH9)在食管鳞癌组织中的表达及临床意义。方法回顾性分析唐山市人民医院2014年1月至2016年12月收治的113例食管鳞癌根治性手术患者的临床资料,采用免疫组织化学染色法检测SRF和MYH9在食管鳞癌组织和相应癌旁组织中的表达,分析SRF和MYH9在食管鳞癌组织中的差异表达与临床病理特征的相关性。结果免疫组织化学染色结果显示,SRF在食管鳞癌组织中的阳性表达率为42.48%(48/113),显著高于癌旁组织的6.25%(2/32),两组比较差异有统计学意义(χ^2=14.487,P<0.05);MYH9的阳性表达率为57.52%(65/113),显著高于癌旁组织的25.00%(8/32),两组比较差异有统计学意义(χ^2=10.551,P<0.05)。SRF和MYH9的表达水平与食管鳞癌的分化程度、淋巴结转移和TMN分期关系密切(P均<0.05);SRF与MYH9的阳性表达呈正相关性(r=0.521,P<0.05)。SRF和MYH9双阴性表达组、SRF和MYH9单阳性表达组、SRF和MYH9双阳性表达组中位生存期分别为30、20、12个月,双阴性、单阳性表达组与双阳性表达组比较,差异均有统计学意义(χ^2值分别43.855、17.799,P均<0.001),双阴性与单阳性表达组比较差异无统计学意义(χ^2=2.787,P=0.095)。结论SRF和MYH9在食管鳞癌组织中高表达,SRF和MYH9双阳性表达患者预后不佳。

Objective To investigate the expression and clinical significance of serum response factor(SRF)and myosin heavy chain 9(MYH9)in esophageal squamous cell carcinoma(ESCC).Methods The clinical data of 113 patients with esophageal squamous cell carcinoma treated in Tangshan people′s Hospital from January 2014 to December 2016 were retrospectively analyzed.Immunohistochemical staining was used to detect the expression of SRF and MYH9 in esophageal squamous cell carcinoma and corresponding adjacent tissues,and the correlation between the differential expression of SRF and MYH9 and clinicopathological features was analyzed.Results Immunohistochemical staining showed that the positive expression rate of SRF in esophageal squamous cell carcinoma was 42.48%(48/113),which was significantly higher than that of 6.25%(2/32)in adjacent tissues(χ^2=14.487,P<0.05).The positive expression rate of MYH9 was 57.52%(65/113),which was significantly higher than that of 25.00%(8/32)in adjacent tissues(χ^2=10.551,P<0.05).The expression levels of SRF and MYH9 were closely related to the differentiation,lymph node metastasis and TMN stage of ESCC(all P<0.05).The positive expression of SRF had a correlation with positive expression of MYH9(r=0.521,P<0.05).The median survival time of SRF and MYH9 double negative expression group,SRF and MYH9 single positive expression group,SRF and MYH9 double positive expression group were 30,20 and 12 months respectively.There were significant differences between the double negative,single positive expression groups and double positive expression groups(χ^2 values were 43.855,17.799,all P<0.001),but there was no significant difference between the double negative and single positive expression groups(χ^2=2.787,P=0.095).Conclusion SRF and MYH9 may be one of potential targets for diagnosis and treatment of esophagus cancer.