桔小实蝇flightin基因RNAi载体构建

Construction of Interference Vector of Bactrocera dorsalis(Hendel)flightin Gene

以云南省林业和草原科学院森保所室内饲养的桔小实蝇为材料,采用RT-PCR技术,克隆桔小实蝇(Bactrocera dorsalis Hendel.)flightin的基因片段,并以此为靶标,设计有效的干扰片段,以线虫RNAi干扰载体L4440为载体,构建桔小实蝇flightin基因RNAi载体。通过PCR、酶切检测及测序,证明表达载体构建正确。本研究为通过RNAi研究桔小实蝇flightin的基因功能奠定了基础。

The sequence of flightin in Bactrocera dorsalis(Hendel)was cloned in this study by RT-PCR.This flightin sequence was chosen as the target gene.An interference fragment was designed and inserted into the expression vector L4440.Restriction enzyme analysis and DNA sequencing showed that all the recombinant plasmids were according with our design.Our study laid a foundation for the study of the function of flightin gene in B.dorsalis by RNAi.