唾液中丙型肝炎病毒抗体的检测及应用

Detection of hepatitis C virus antibody in saliva and its clinical significance

目的 探讨唾液中丙型肝炎病毒 (hepatitisCvirus,HCV)抗体检测的可行性及其临床应用的意义。方法 应用改进后的酶免疫技术 ,分别对 86例患者外周血和唾液样品中的HCV抗体 (抗 HCV)进行平行检测、对比分析。结果 86例患者中 ,外周血抗 HCV阳性 2 9例 ,其中 2 7例唾液检测抗 HCV也是阳性 ,另外 2例为阴性 ;外周血抗 HCV阴性 57例 ,其唾液检测也均为阴性。与外周血的检测结果相比 ,唾液样品中抗 HCV检测的灵敏度 (93 1 % ,2 7/2 9)、特异性 (1 0 0 % ,57/57)均较好。结论 唾液中抗

Objective To detect hepatitis C virus (HCV) antibody in saliva and find its clinical significance Methods Sera and saliva samples from eighty six patients were parallely detected HCV antibodies (anti HCV) with EIA using a modified protocol Results Twenty nine of 86 patients were positive for anti HCV in sera samples ; and, 27/29 were also anti HCV positive with saliva samples For the 57 anti HCV negative sera, the saliva samples were all negative Comparing with the results of sera, the sensitivity (93 1%,27/29) and specificity (100%,57/57) of salivary detection for anti HCV could be accepted Conclusion If the results of sera are considered as 'golden standard',these results suggest that tests on saliva can be useful in HCV clinical diagnosis and epidemiological studies for estimating the prevalence of HCV in populations