海绵共附生菌Streptomyces sp. LHW2432 的次级代谢产物研究

Study on secondary metabolites from sponge-symbiotic Streptomyces sp.LHW2432

目的从海绵共附生菌Streptomyces sp.LHW2432的发酵产物中发现药用活性分子。方法采用正向硅胶柱、ODS反向柱以及高效液相色谱分离技术,对LHW2432的发酵萃取物进行分离纯化;通过现代波谱技术和文献调研确定化合物结构;利用平板涂布法和微量稀释法,评价化合物对芽胞杆菌、耐甲氧西林金黄色葡萄球菌(MRSA)、耻垢分支杆菌、白色念珠菌和大肠杆菌的抑菌活性。结果从LHW2432发酵物中共分离鉴定了5个化合物:descycloavandulyllavanduquinocin(1)、N-acetyltyramine(2)、phomapyrone C(3)、germicidin A(4)和germicidin I(5)。化合物1对MRSA和耻垢分支杆菌的最小抑菌浓度(MIC)值分别为100和64μg/ml。结论从LHW2432菌中分离得到5个化合物,其中,化合物1是新天然产物,可作为神经保护活性三环咔唑类生物碱的合成前体,其对G+菌有微弱的抑制活性。

Objective To discover the medicinal active molecules from the fermentation extract of sponge-symbiotic Streptomyces sp.LHW2432.Methods Compounds were isolated and purified from the fermentation extract of LHW2432 by silica gel,ODS chromatographic columns,and HPLC.The structures of the compounds were elucidated based on the analyses of modern spectrum technologies and the related literatures research.Through plate coating method and broth microdilution method,the antimicrobial activities were tested by the indicator strains of Bacillus mycoides,methicillin-resistant Staphylococcus aureus(MRSA),Mycobacterium smegmatis,Candida Albicans,and Escherichia coli.Results Five compounds were discovered and their structures were identified as descycloavandulyl-lavanduquinocin(1),N-acetyltyramine(2),phomapyrone C(3),germicidin A(4),and germicidin I(5).Compound 1 showed inhibitory activities against MRSA(MIC,100μg/ml)and M.smegmatis(MIC,64μg/ml),respectively.Conclusion Five compounds were discovered from LHW2432,among which compound 1 was a new natural product and could be used as a precursor of the tricyclic carbazole alkaloids with neuroprotective activity.Moreover,compound 1 showed weak inhibitory activities against gram-positive pathogenic bacteria.