复方滋阴颗粒对去卵巢大鼠性激素和氧化应激水平的影响

Effects of compound Ziyin granule on sex hormones and oxidative stress levels of ovariectomized rats

目的研究复方滋阴颗粒对去卵巢大鼠性激素和氧化应激水平的影响。方法实验分为模型组、六味地黄丸组、戊酸雌二醇组、滋肾养阴汤剂组和复方滋阴颗粒(低、中、高剂量)组、正常组和假手术组,给药8周,检测血清雌二醇(E2)、促卵泡素(FSH)、促黄体生成素(LH)、总超氧化物歧化酶(T-SOD)、丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-Px)水平。结果与模型组比较,复方滋阴颗粒(简称“颗粒”)中、高剂量可显著升高E2水平,中剂量可降低LH水平,低、中、高剂量均可降低FSH水平;颗粒中、高剂量可显著升高T-SOD、GSH-Px活性,降低MDA水平,低剂量则可升高T-SOD活性并降低MDA水平(P<0.05或P<0.01)。与戊酸雌二醇组比较,低剂量可明显升高T-SOD活性(P<0.05);与汤剂组比较,颗粒中、高剂量可明显升高GSH-Px活性(P<0.05)。结论复方滋阴颗粒对去卵巢围绝经期模型大鼠垂体-卵巢轴的性激素分泌功能的异常有逆转作用,并提高其抗氧化能力。

Objective To investigate the effect of compound Ziyin granule(CZG)on sex hormones and oxidative stress levels of ovariectomized rats.Methods The experiment was divided into model group,Liuwei Dihuang pill group,estradiol valerate group,Zishen Yangyin Decoction group,CZG(low,medium,high dose)group,ormal group and sham operation group.After administration for 8 weeks,serum estradiol(E2),follicle stimulation hormone(FSH),luteotropic hormone(LH),totalsuperoxide dismutase(T-SOD),malonaldehyde(MDA)and glutathione peroxidase(GSH-Px)were measured.Results Compared with the model group,E2 was significantly raised at medium and high doses of CZG,LH was decreased at medium dose,and FSH was reduced at low,medium and high dose;medium and high dose of CZG were capable of remarkably increasing T-SOD,GSH-Px and decreasing MDA,and low dose raised T-SOD and reduced MDA(P<0.05 or P<0.01).Compared with estradiol valerate group,low dose of CZG significantly increased T-SOD(P<0.05).Compared with decoction group,GSH-Px was remarkably raised at medium and high dose(P<0.05).Conclusion The CZG could reverse the abnormal function of sex hormone secretion in the pituitary-ovary axis of the ovariectomy perimenopausal model rat and improve its antioxidant capacity.