Khai 岛和Pathiu 岛珊瑚礁沉积物细菌多样性及细菌粗提物延缓秀丽隐杆线虫衰老活性研究

Studies on bacterial diversity in coral reef sediments in Khai Island and Pathiu Island and bacterial crude extract retards aging activity of Caenorhabditis elegans

在Khai岛和Pathiu岛珊瑚礁生长海域采集2份珊瑚礁沉积物样品,将样品预处理后采用6种分离培养基进行分离培养,获得活性海洋细菌菌株,通过PCR扩增和16s rRNA基因序列测定,分析物种多样性,并构建系统发育树。以模式生物秀丽隐杆线虫为模型考察菌株发酵提取物延缓衰老的作用。实验结果表明,从4种样品中分离鉴定出22株细菌,分别属于11目13科14属。16S rRNA基因序列相似性低于98.65%的细菌有2株,疑似为潜在新种。GXIMD014(Vibrio galatheae)和GXIMD112(Kocuria kristinae)的500μg·mL–1细菌粗提物具有延缓线虫衰老的活性,能不同程度的延长线虫寿命,提高线虫产卵量、运动能力、热激损伤能力、抗氧化损伤能力、体内酶活性以及改善虫体表皮形态。Khai岛和Pathiu岛珊瑚礁沉积物细菌具有较高的物种多样性,蕴藏着丰富的微生物资源,且部分细菌菌株具有延缓衰老的生物活性。

Two coral reef sediment samples were collected from the coral reef growth areas of Khai Island and Pathiu Island After the samples were pretreated,six kinds of separation medium were adopted to obtain the active Marine bacterial strains.Through PCR amplification and 16s rRNA gene sequencing,species diversity was analyzed and phylogenetic trees were constructed.The effect of fermentation extract of Caenorhabditis elegans strain on senescence was investigated.The results showed that 22 strains of bacteria were isolated and identified from four samples,belonging to 11 orders,13 families and 14 genera.There were two strains of bacteria with 16S rRNA gene sequence similarity less than 98.65%,which were suspected to be potential new species.The crude extracts of 500μg·mL–1 bacteria from GXIMD014(Vibrio galatheae)and GXIMD112(Kocuria kristinae)have the activity of delaying the aging of Caenorhabditis elegans,and can prolong the life of Caenorhabditis elegans to different degrees,improve the oviposition,motility,heat-induced injury,ability to resist oxidative damage,enzyme activity in vivo,and improve the epidermal morphology of Caenorhabditis elegans.Coral reef sediment bacteria of Khai Island and Pathiu Island have high species diversity and rich microbial and bacterial resources,and some bacterial strains have anti-aging biological activity.