紫外分光光度法测定草阿魏中总香豆素含量

The content of the total coumarins was determined by UV spectrophotometry in Cao Giantfennel

目的:建立草阿魏中总香豆素含量的测定方法。方法:以7-羟基香豆素为对照品,采用紫外分光光度法测定草阿魏中总香豆素含量。结果检测波长为326nm,7-羟基香豆素浓度在4.1~14.3μg·mL^-1范围内与吸光度呈良好线性关系(γ=0.9999),平均回收率为101.82%(RSD=1.59%,<2%)。结论:本方法简便易行,结果稳定可靠,可用于草阿魏的质量控制。

Objecfive To establish the method for content determination of total coumarins in Cao Giantfennel.Methods The content of the total coumarins was determined by UV spectrophotome-try.Results The optimal detective wavelength was 326 nm for the total coumarins.Satisfactroy linear relationship with the absorbability was achieved when the mass concentration of osthole was within the range of 4.1~14.3μg·mL^-1(γ=0.9999).The average recovery rate was101.82%(RSD=1.59%,<2%).Conclusion The method is simple,accurate and sensitive,and it can be used for the quality control of Cao Giantfennel and its preparation.