离子色谱法和高效液相色谱法测定化妆品中的丁卡因及其盐类

Determination of tetracaine and its salts in cosmetics by IC and HPLC

建立了化妆品中丁卡因及其盐类的离子色谱和高效液相色谱分析方法,并对两种检测方法进行了比对分析。采用离子色谱法,丁卡因及其盐类的检出限和定量限分别为10和25 mg/kg,方法平均回收率为87.6%~102.7%,相对标准偏差为1.1%~4.7%。采用高效液相色谱法,丁卡因及其盐类的检出限和定量限分别为2和5 mg/kg,方法平均回收率为89.5%~105.6%,相对标准偏差为1.4%~5.8%。对于疑似阳性样品,进一步采用高效液相色谱-串联质谱法进行确证分析。经比较,两种方法检测化妆品中的丁卡因及其盐类的色谱保留时间均在5 min以内,方法学数据均满足分析要求。

Two analytical methods were developed for the determination of tetracaine and its salts in cosmetics based on ion chromatography(IC)and high-performance liquid chromatography(HPLC).The two detection methods were compared.The limit of detection(LOD)and quantitation(LOQ)of tetracaine were 10 and 25 mg/kg for the IC method.The average recoveries ranged from 87.6%to 102.7%with the relative standard deviations(RSDs)of 1.1%-4.7%.The LOD and LOQ were 2 and 5 mg/kg for the HPLC method.The average recoveries ranged from 89.5%to 105.6%with the RSDs of 1.4%-5.8%.The suspected positive samples were subjected to further verification by high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).The chromatographic retention time of tetracaine and its salts was both within 5 min and the experimental data of the two methods met the performance requirements.