双波长HPLC同时测定生地黄中6种化合物的含量

Simultaneous Determination of 6 Compounds in Rehmanniae Radix by Dual-wavelength HPLC

目的采用双波长HPLC同时测定生地黄中6种化合物(梓醇、地黄苷D、地黄苷A、益母草苷、毛蕊花糖苷、异类叶升麻苷)的含量。方法菲罗门Neptune C18色谱柱(4.6 mm×250 mm,5μm);流动相:乙腈-0.1%磷酸溶液,梯度洗脱;流速:1.0 mL·min^-1;柱温30℃;检测波长203 nm(梓醇、地黄苷D、地黄苷A、益母草苷),334 nm(毛蕊花糖苷、异类叶升麻苷)。结果6种化合物在各自的范围内呈现良好的线性关系(r>0.9990),3个浓度水平回收率96.33%~98.55%,RSD为0.97%~1.45%。结论该法简单快捷、稳定可靠,准确率高,重复性好,可用于生地黄质量评价。

OBJECTIVE To establish a dual-wave length HPLC method for the determination of 6 components in Rehmanniae Radix(including catalpol,rehmannioside D,rehmannioside A,leonuride,acteoside and isoacteoside).METHODS Neptune C18 column(4.6 mm×250 mm,5μm)was selected for separation with acetonitrile-0.1%phosphoric acid solution as the mobile phase for gradient elution,the flow rate was 1.0 mL·min^-1,the column temperature was maintained at 30℃and the detection wavelength was set at 203 nm for catalpol,rehmannioside D,rehmannioside A and leonuride,334 nm for acteoside and isoacteoside.RESULTS Six components showed good linear relationships(r>0.9990)in their own ranges.The average recoveries at three levels ranged from 96.33%to 98.55%with RSD of 0.97%to 1.45%.CONCLUSION This method is simple,accurate,reliable,stable and reproducible,which can be used for the quality control of Rehmanniae Radix.