双氢青蒿素和吉非替尼联用对肺癌细胞侵袭和迁移能力的影响

Effect of the cellular migration and invasion of DHA and gefitinib in combination in lung adenocarcinoma cells

探讨了双氢青蒿素(DHA)与吉非替尼联用对肺癌A549细胞侵袭和迁移能力的影响并探讨其机制。通过CCK8方法检测DHA吉非替尼联合用药对细胞增殖抑制作用。TUNEL方法检测A549细胞凋亡情况;Transwell实验检测A549细胞的侵袭和迁移能力;蛋白印迹技术检测A549细胞信号通路AKT/EGFR蛋白的表达。结果与吉非替尼单独用药组比较,联合用药组能显著抑制A549细胞增殖,增强吉非替尼诱导A549细胞的凋亡作用;能明显抑制A549细胞的侵袭和迁移并显著地降低了p-AKT、p-EGFR蛋白的表达。DHA与吉非替尼联用能显著抑制A549细胞的侵袭和迁移能力,其作用机制可能与AKT/EGFR信号通路调节有关。

Explore the cellular migration and invasion of DHA and gefitinib in combination in lung adenocarcinoma A549 cells and the possible mechanism.Counting kit 8 assay was used to evaluate cell viability of DHA and gefitinib in A549 cells;Transwell assays were performed to investigate whether DHA and gefitinib may induce cellular migration and invasion in A549 cells;the terminal deoxynucleotidyl transferase dUTP nick-end labeling assays were used to evaluate the effect of apoptosis in A549 cells;western blot analysis was used to detect the expression levels of target proteins including signaling pathway proteins(Akt/EGFR).Compared with gefitinib alone,DHA can enhance the sensitivity of gefitinib in A549 cells,DHA increase the apoptosis of gefitinib,not only could DHA increase the apoptosis of gefitinib,but also DHA can enhance the invasion and migration of A549 cells.DHA combined with gefitinib significantly decreased the phosporlated levels of AKT and EGFR protein.In conclusion,this possible mechanism of DHA-increased sensitivity of cells to gefitinib is related with the regulation of AKT/EGFR signaling pathways.