响应面优化龙葵果多糖提取工艺及抗氧化活性的研究

Optimization Extraction and Antioxidant Activity of Polysaccharide from Solanum Nigrum Fruit by Response Surface Methodology

利用响应面法优化龙葵果多糖提取工艺。单因素实验研究提取时间、乙醇浓度以及料液比对龙葵果多糖提取工艺的影响,在此基础上,应用Design-Expert8.0.6建立数学模型,进行3因素3水平的响应面分析,并对龙葵果多糖进行了抗氧化的体外活性实验。结果表明:提取龙葵果多糖的最佳条件为:提取时间5 h、乙醇浓度94%、料液比1:20 g/mL,此条件下进行重复实验,其龙葵果中多糖得率为4.07 mg/g。在抗氧化实验中,龙葵果多糖对DPPH自由基和·OH均有一定的清除能力,其清除DPPH自由基和·OH的半抑制浓度(IC50)别为65.43μg/mL和0.33 mg/mL。

Using response surface method to optimize the extraction process of polysaccharide from Solanum nigrum fruit.Single factor test to study the effect to extraction time ethanol concentration and feed-to-liquid ration the extraction process of Solanum nigrum fruit polysaccharides.On this basis,design-expert 8.0.6 was used to establish a mathematical model for three factor three level response surface analysis,the antioxidant activity of the polysaccharides was tested.The results showed that the optimum conditions were as follows:extraction time 5 h,ethanol concentration 94%,feed-liquid ratio 1:20.The yield of polysaccharide in Solanum nigrum fruit was 4.07 mg/g.In the antioxidant experiment,Solanum nigrum fruit polysaccharide has a certain scavenging ability to DPPH free radical and·OH,and the IC50 of scavenging DPPH free radical and·OH are 65.43μg/mL and 0.33 mg/mL respectively.