姜黄素通过内质网应激信号通路诱导肝癌SMMC-7721细胞凋亡的作用及对细胞活性影响分析

Effect of curcumin on apoptosis of liver cancer SMMC-7721 cells induced by endoplasmic reticulum stress signaling pathway and its effect on cell viability

目的分析姜黄素通过内质网应激信号通路诱导肝癌SMMC-7721细胞凋亡的作用及对细胞活性影响。方法选取2017年4月~2018年3月期间为研究时间段,以此期间购置的肝癌SMMC-7721细胞为试验样本,利用不同浓度姜黄素处理试验样本,先经流式细胞仪检测细胞凋亡情况,后经CCK8法分别在试验开始后24 h检测其细胞活力,再经流式细胞仪检测肝癌细胞ROS含量。结果姜黄素浓度越高,肝癌细胞存活率呈逐步下降趋势,同空白对照样本比较,有显著性差异(P<0.05);姜黄素浓度越高,MFI值呈逐步升高趋势,同空白对照样本比较,有显著性差异(P<0.05);不同浓度姜黄素处理的试验样本细胞凋亡率,呈逐渐上升趋势,与空白对照样本比较,有显著性差异(P<0.05)。结论利用姜黄素可有效地抑制肝癌细胞活性,诱导肝癌细胞凋亡。

Objective To analyze the effect of curcumin on apoptosis of liver carcinoma cell line SMMC-7721 through endoplasmic reticulum stress signaling pathway and its effect on cell viability.Methods The period from April 2017 to March 2018 was selected as the study period.The liver cancer SMMC-7721 cells purchased during this period were used as test samples.The test samples were treated with different concentrations of curcumin,and the apoptosis of cells were first detected by flow cytometry.Then the cell viability was detected by CCK8 method 24 h after the start of the experiment.And the ROS content of liver cancer cells was detected by flow cytometry.Results The higher the concentration of curcumin,the lower the survival rate of liver cancer cells.Compared with that of the blank control samples,the data showed significant difference(P<0.05).The higher the curcumin concentration,the higher the MFI value.Compared with that of the blank control samples,the data showed significant difference(P<0.05).The apoptosis rate of the samples treated with different concentrations of curcumin showed a gradual increase trend,which was significantly different from that of the blank control group(P<0.05).Conclusion Curcumin can effectively inhibit the activity of liver cancer cells and induce apoptosis of liver cancer cells.