Stathmin、P16、P63、Ki-67在宫颈上皮内瘤变中的表达及意义

Expression and significance of Stathmin,P16,P63,Ki-67 in cervical intraepithelial neoplasia

目的探讨Stathmin、P16、P63、Ki-67在子宫颈鳞状上皮内瘤变(CIN)中的表达及意义。方法收集2017年6月~2018年6月间在我院宫颈活检及利普刀锥切标本396例,其中慢性宫颈炎标本74例作为对照组,CINⅠ级标本113例、CINⅡ级标本122例、CINⅢ级标本87例作为观察组。采用免疫组化(IHC)检测Stathmin、P16、P63、Ki-67的表达情况。结果Stathmin蛋白在慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ的表达分别为5.41%(4/74)、15.93%(18/113)、38.53%(47/122)、88.51%(77/87),其中观察组与对照组比较,差异有统计学意义(P<0.01)。P16蛋白在对照组和观察组的表达分别为12.16%(9/74)、85.84%(97/113)、100.00%(122/122)、100.00%(87/87),观察组与对照组比较,差异有统计学意义(P<0.01)。P63蛋白在对照组和观察组的表达分别为6.76%(5/74)、66.37%(75/113)、97.54%(119/122)、100.00%(87/87),观察组分别与对照组比较,差异有统计学意义(P<0.01),Ki-67在对照组和观察组的表达分别为24.32%(18/74)、75.22%(85/113)、100.00%(122/122)、100.00%(87/87),观察组与对照组比较,差异有统计学意义(P<0.01)。结论联合检测Stathmin、P16、P63、Ki-67有助于鉴别宫颈的良性病变和宫颈上皮内瘤变,并可区分宫颈上皮内瘤变的低度病变和高度病变,以准确指导临床治疗。

Objective To investigate the expression and significance of Stathmin,P16,P63,Ki-67 in cervical squamous intraepithelial neoplasia(CIN).Methods 396 cases of cervical biopsy and lip knife cone cutting specimens were collected in our hospital from June 2017 to June 2018,including 74 cases of chronic cervicitis specimens as a control group,113 cases of CIN I grade specimens,122 cases of CIN II grade specimens,87 cases of CINⅢspecimens were used as an observation group.Immunohistochemistry(IHC)was used to detect the expression of Stathmin,P16,P63 and Ki-67.Results The expression of Stathmin protein in chronic cervicitis,CINⅠ,CINⅡ,and CINⅢwas 5.41%(4/74),15.93%(18/113),38.53%(47/122),and 88.51%(77/87),and there were statistically significant differences between the observation group and control group(P<0.01).There were statistically significant differences in the expression of P16 protein between the observation group[12.16%(9/74)]and the observation group[85.84%(97/113),100.00%(122/122),and 100.00%(87/87)](P<0.01).There were statistically significant differences in the expression of P63 protein between the control group[6.76%(5/74)]and the observation group[66.37%(75/113),97.54%(119/122),and 100.00%(87/87),respectively](P<0.01).The expression of Ki-67 was 24.32%(18/74)in the control group,75.22%(85/113),100.00%(122/122),and 100.00%(87/87)in the observation group,and the difference between the two groups was statistically significant(P<0.01).Conclusion The joint detection of Stathmin,P16,P63 and Ki-67 is helpful to identify benign cervical lesions and cervical intraepithelial neoplasia,and can distinguish between low-grade and high-grade cervical intraepithelial neoplasia to accurately guide clinical treatment.