AIB1在肺腺癌中的表达及其临床意义

AIB1 expression and its clinical significance in lung adenocarcinoma

目的探讨乳腺癌扩增基因1(amplified in breast cancer 1,AIB1)在肺腺癌中的表达及其与患者预后的关系,探讨AIB1促进肺腺癌细胞生长转移的机制。方法收集2015年1月1日至2018年12月31日首都医科大学宣武医院收治的肺腺癌患者102例。通过免疫组化检测肺腺癌组织中AIB1的表达,分析AIB1表达与患者预后相关性。通过质粒转染使肺腺癌细胞株A549过表达AIB1,观察AIB1过表达对细胞生长、细胞周期及划痕愈合能力的影响。结果102例患者中,AIB1高表达者60例,低表达者42例,死亡患者AIB1的表达显著高于存活患者(P=0.001),AIB1高表达者累计生存率显著降低(P<0.001)。与转染空质粒相比,转染AIB1的A549细胞生长增快,细胞周期进程加速,划痕愈合能力增强(P<0.05)。结论AIB1可以促进肺腺癌细胞生长、增殖和转移,并与肺腺癌患者的不良预后相关。

Objective To investigate the expression of amplified in breast cancer(AIB1)in lung adenocarcinoma and its relationship with prognosis,and analyze the mechanism of AIB1 promoting lung adenocarcinoma growth and metastasis with in vitro experiments.Methods The expression of AIB1 in lung adenocarcinoma tissue was detected by immunohistochemistry,and the correlation between AIB1 and prognosis was analyzed.Overexpression of AIB1 in lung adenocarcinoma cells by plasmid transfection to study the effect of AIB1 on the cell proliferation,cell cycle and migration.Results There were high AIB1 expression in 60 patients and low AIB1 expression in 42 patients.The expression of AIB1 in the dead patients was significantly higher than that in the surviving patients(P=0.001),and the cumulative survival rate of those with high AIB1 expression was significantly reduced(P<0.001).Compared with transfecting empty plasmids,A549 cells transfected with AIB1 grew faster,cell cycle progression accelerated,and migration and healing capabilities enhanced(P<0.05).Conclu⁃sions AIB1 can promote the growth,proliferation and metastasis of lung adenocarcinoma cells and is associated with poor prognosis in the patients with lung adenocarcinoma.