食管鳞癌组织中LIV-1蛋白的表达及预后分析

The expression of LIV-1 protein and prognosis analysis in tissues of esophageal squamous cell carcinoma

目的比较LIV-1蛋白高表达组与低表达组中食管鳞癌患者临床特征及预后。方法收集2012年2月至2016年8月139例(T3N0-3M0)食管鳞癌患者根治术后石蜡组织为研究对象,36例癌旁正常食管上皮组织作为对照,用免疫组织化学方法检测锌离子转运体LIV-1蛋白的表达情况;根据LIV-1蛋白表达水平不同分成高表达组、低表达组,并进行中位随访时间31个月的随访。结果(1)LIV-1蛋白低表达患者66例(47.5%),高表达患者73例(52.5%),两组间年龄、性别、肿瘤位置、分化程度、淋巴结状态差异无统计学意义(P>0.05);(2)在正常食管上皮组织、食管鳞癌组织中,LIV-1蛋白高表达率分别为11.1%、52.5%;(3)LIV-1蛋白低表达组患者的总生存(OS)与无病生存期(DFS)明显好于高表达组。(4)Cox回归多因素分析提示,LIV-1蛋白高表达组的死亡风险显著高于低表达组。结论LIV-1蛋白高表达是食管鳞癌的独立危险因素。LIV-1蛋白表达水平与食管鳞癌患者分化程度、淋巴结状态无关。

Objective To compare the patients′clinical characteristics and prognosis in the high and lowexpression groups of LIV-1 protein with esophageal squamous cell carcinoma.Methods139 tissues from patients ofesophageal cancer(T3 N0-3 M0)post to the radical surgery were collected in the pathological department fromFebruary 2012 to August 2016 and 36 cases of normal esophageal epithelium tissues adjacent to the cancer wereselected as control.The expression of zinc transporter LIV-1 protein was detected using immunohistochemistry.Highand low expression groups were selected according to the different levels and the median follow-up time was 31 months.Results(1)The number of patients with low expression was 66(47.5%)where as the high expression was73(52.5%).Between the two groups,there were no statistically significant differences among age,gender,locationof tumor,level of differentiation,and status of lymph node(P values are greater than 0.05);(2)the high expressionrates of LIV-1 protein were 11.1%and 52.5%in normal esophageal epithelial and esophageal squamous cellcarcinoma tissues,respectively.(3)The overall survival(OS)and disease-free survival(DFS)of patients in the lowexpression group were significantly better than those in the high expression group.(4)Multivariate analysis of COXregression suggested that the risk of death was significantly increased in the high expression group compared with theother.ConclusionsThe high expression of LIV-1 protein is an independent prognostic factor of esophagealsquamous cell carcinoma.The expression level of LIV-1 protein has no correlation with the degree of differentiationand lymph node status of esophageal squamous cell carcinoma.