基于UPLC-LTQ Orbitrap MS的益气降脂颗粒化学组成分析

Chemical Composition Analysis of Yiqi Jiangzhi Granules Based on UPLC-LTQ Orbitrap MS

建立了中药复方复杂成分快速鉴定的高效分析方法,并应用该方法对复方中药制剂益气降脂颗粒中的化学成分进行分析。通过超高效液相色谱-线性离子阱/静电场轨道阱组合式高分辨质谱(UPLC-LTQ Orbitrap MS)扫描提供的化合物精确相对分子质量、多级碎片离子信息,与对照品的相对保留时间和质谱数据进行比对,并结合相关参考文献,实现对化合物结构的准确鉴定。共鉴定了益气降脂颗粒中97个化学成分,主要包括黄酮类、生物碱类、酚酸类、蒽醌类、三萜类、倍半萜类及其他类成分,其中小麦黄素、小麦黄素-7-O-葡萄糖苷、8-乙酰基-7-羟基-4-甲基香豆素、3-乙酰基香豆素为首次从益气降脂颗粒组方药味中鉴定得到。对益气降脂颗粒中多种化学成分的系统分析可以为其药效物质基础及质量控制研究提供科学理论依据。

In order to comprehensively understand the chemical composition of Yiqi Jiangzhi Granules,ultra-high performance liquid chromatography-linear ion trap/electro-static orbitrap combined high resolution mass spectrometry(UPLC-LTQ Orbitrap MS)was applied.By comparing the exacted molecular mass,retention time and mass spectrometry fragment ion information of each component to reference substance and related literature,a total of 97 chemical composition were identified,including flavonoids,alkaloids,phenolic acids,anthraquinones,triterpenoids,sesquiterpenoids and other components.Besides,tricin,tricin-7-O-glucoside,8-acetyl-7-hydroxy-4-methylcoumarin and 3-acetylcoumarin were identified from the ten medicinal ingredients in Yiqi Jiangzhi prescription for the first time.Meanwhile,this study can provide a reference for the research of Yiqi Jiangzhi Granules quality control and pharmacodynamics development.