花生CONSTANS-Like(COL)家族基因的克隆与表达分析

Cloning and expression analysis of CONSTANS-Like(COL)family genes in peanut(Arachis hypogaea L.)

开花是植物从营养生长到生殖生长的重要过程,CONSTANS-Like(COL)基因是植物光周期诱导开花途径中的关键转录因子,但是在花生中的具体功能尚不清楚。本研究通过生物信息学的方法对花生COL基因家族成员序列特征、系统进化关系、基因结构、保守结构域及表达模式进行分析,最终克隆了17个AhCOL基因(AhCOL1-Ah⁃COL17)。花生17个AhCOL基因氨基酸长度范围为327aa^617aa,等电点(pI)均小于7,在蛋白质序列的氮端与碳端均含有保守的BBX和CCT结构域。此外,不同组织中的基因表达模式分析,表明多数AhCOL基因在叶片中的表达量显著高于其他组织。不同时期的表达量分析表明多数AhCOL基因的表达量从苗期到开花期呈现递增。本研究为研究花生AhCOL基因的潜在功能奠定了重要基础。

Flowering is an important process from vegetative growth to reproductive growth,CONSTANS-Like(COL)genes belong to the critical transcription factor genes in plant photoperiod induced flowering pathway,but their functional detail in peanut is still unclear.In this study,we illustrated the sequence characteristics,phylogenetic relationship,gene structure,conserved domain and expression pattern of COL family genes in peanut by bioinformatics analysis,and cloned 17 AhCOL genes(AhCOL1-AhCOL17).The amino acid length of 17 AhCOL genes ranged from 327 aa to 617 aa in peanut genome,the isoelectric points(pI)were less than 7,and the conserved domains of BBX and CCT existed in N-terminal and C-terminal of COL protein sequence,respectively.Furthermore,the expression pattern analysis of 17 AhCOL in different tissues showed that the relative expression of majority Ah⁃COL genes in leaves was significantly higher than in another tissue.Meanwhile,the expression levels of most Ah⁃COL genes presented increasing from seedling to flowering at different stage during peanut growth.This study laid an improtant foundation for studying the potential function of AhCOL genes in peanut.