聚-γ-谷氨酸对柑橘苗木性状和GABA的影响

Effects of poly-γ-glutamic acid on seedlings trait and GABA of citrus

通过株高、冠径及酶活的测定研究聚-γ-谷氨酸(PGA)水溶液灌根对柑橘苗木个体性状和叶片中抗性相关酶的影响,并采用亲水相互作用色谱(HILIC LC-MS/MS)分析了PGA灌根前后叶片中200个靶向代谢物含量的变化。结果表明:PGA处理能促进苗木株高、冠径和嫁接苗分枝数的增加;PGA处理后,柑橘叶片中超氧化物歧化酶(SOD,抗氧化物保护酶)、过氧化物酶(POD,参与过氧化产物的清理)和多酚氧化酶(PPO,酚类物质代谢的参与酶)的活性增加;PGA处理后,柑橘叶片中L-谷氨酸含量下降73.2%倍,而γ-氨基丁酸(GABA)含量增加了1.6倍,N6-甲基腺嘌呤含量提高2.3倍。靶向代谢物分析获得109个代谢物的定量结果,其中,13种代谢物对照与处理的差异达到显著水平。

The effects of poly-γ-glutamic acid(PGA)aqueous solution irrigation on seedlings trait and resistance-related enzymes in leaves of citrus were determined by measuring plant height,crown diameter and enzyme activity.HILIC LC-MS/MS was used to analyze the changes of 200 target metabolites in leaves after irrigation of PGA.The results showed that PGA treatment increased the height,crown diameter and branch number of seedlings.The activities of superoxide dismutase(SOD),peroxidase(POD)and polyphenol oxidase(PPO)in citrus leaves were increased after PGA treatment.The content of L-glutamate in citrus leaves decreased by 73.2%,while the content ofγ-aminobutyric acid(GABA)increased by 1.6 times.The content of N6-methyl adenine increased by 2.3 times.The results of quantities of 109 metabolites analyzed by targeted metabolite showed that the difference of 13 metabolites between control and treatment was significant.