β-氨基丁酸结合碳酸氢钠对采后损伤山药愈伤的影响

Effect ofβ-aminobutyric acid combined with sodium bicarbonate on callus of yam

为研究化学诱抗剂对采后机械损伤山药愈伤效果及抗病性的影响,采用1 mmol/Lβ-氨基丁酸(BABA)结合NaHCO3复合处理人工损伤山药,在35℃下(RH 85%)愈伤7 d。通过测定愈伤期间损失率、腐烂程度、木质素、酚类物质和超氧化物含量来评价愈伤效果。结果显示,人工机械损伤山药经1 mmol/L BABA结合NaHCO3复合处理后,山药的腐烂程度明显降低,能快速促进伤口部位木质素和酚类物质的积累,大幅提升超氧化物含量。由此表明,BABA结合NaHCO3复合处理能有效抑制山药愈伤期间病原菌侵染,促进损伤山药愈伤结构快速形成。

In order to study the effect of chemical inducers on the curing development and disease resistance of mechanical damaged yam,the mixture of 1 mmol/L BABA and NaHCO3 was used to treat artificially damaged yam.The callus was cultured at 35℃for 7 days(RH 85%).The weight loss rate,decay degree,lignin,phenols and superoxide during the callus period were measured to evaluate the effect of curing treatment.The results showed that the combined treatment of BABA and NaHCO3 significantly reduced the decay degree of damaged yam,promoted the accumulation of lignin and phenolic substances in the wounded site,and significantly increased the content of superoxide.The results showed that the combination of BABA and NaHCO3 could effectively inhibit the infection of pathogens and promote the rapid formation of wound periderm of yam.