碘油与葡聚糖微球栓塞兔肝VX2移植瘤疗效对比

Comparison of efficacies of iodized oil and dextran microspheres in embolism of rabbit hepatic VX2 transplantation tumors

目的用碘油与葡聚糖微球分别行兔肝VX2移植瘤栓塞,通过对比CT灌注值及免疫组织化学CD34、血管内皮生长因子(vascular endothelial growth factor,VEGF)表达情况,比较2种栓塞剂的疗效。方法用碘油与葡聚糖微球分别行兔肝VX2移植瘤栓塞,通过对比CT灌注值以及免疫组织化学CD34、血管内皮生长因子(vascular endothelial growth factor,VEGF)表达情况,比较2种栓塞剂的疗效。结果①栓塞后葡聚糖微球组组织血流量(blood flow,BF)、组织血容量(blood volume,BV)、肝动脉供血分数(hepatic arterial fraction,HAF)、肝动脉灌注量(hepatic arterial perfusion,HAP)均小于碘油组,而门静脉灌注量(portal vein perfusion,PVP)大于碘油组。②免疫组织化学研究发现碘油组及葡聚糖微球组在瘤周的正常肝实质内未见或仅仅少量CD34及VEGF染色;肿瘤区域内碘油组CD34及VEGF的表达量大于葡聚糖微球组,2组之间的微血管密度(microvascular density,MVD)值差异有统计学意义(P<0.05)。结论葡聚糖微球栓塞效果优于碘油,是一种理想的栓塞剂。

Objective The embolization of rabbit hepatic VX2 transplantation tumors was carried out by iodized oil and dextran microspheres respectively.The curative effect of the two kinds of embolization agents was determined by the comparison of CT perfusion values and the expressions of immunohistochemical CD34 and vascular endothelial growth factor(VEGF).Methods Fifty tumor-bearing rabbits were randomly divided into iodized oil group and dextran microsphere group.CT perfusion scans were carried out in 14 days after liver VX2 tumor planting in the rabbits.Hepatic arterial embolization was performed on the 15th day after the planting of rabbit liver VX2 tumors.CT perfusion scan was carried out in 14 days after embolization to observe the change of CT perfusion values.After these tumor-bearing rabbits were killed,the rabbit liver VX2 tumors were isolated,and then immunohistochemical staining was used to observe the expressions of CD34 and VEGF.Results①Blood flow,blood volume,hepatic arterial fraction and hepatic arterial perfusion of dextran microsphere group were smaller than those of iodized oil group,and portal vein perfusion of dextran microsphere group was larger than that of iodized oil group.②Immunohistochemical studies showed that no or only a small amount of CD34 and VEGF staining was found in the normal liver parenchyma around the tumors in the iodized oil group and the dextran microsphere group.The expressions of CD34 and VEGF in tumor area in iodized oil group were greater than those in dextran microsphere group,and the differences of microvascular density(MVD)values between two groups were statistically significant(P<0.05).Conclusion The embolization effect of dextran microsphere is better than that of iodized oil,so it is an ideal embolization agent.