红曲中酸型Monacolin K提取工艺研究

Study on the Extraction Process of Acid Type Monacolin K from Monascus

目的优选红曲中提取酸型Monacolin K的最佳提取工艺。方法采用单因素试验,对酸型Monacolin K提取工艺的影响因素进行了考察。以酸型Monacolin K的提取量和酸型Monacolin K占Monacolin K两种形态(酸型和内酯型)的比例为评价指标,考察了提取工艺中提取溶剂pH值、提取溶剂、料液比、提取时间、提取温度等因素对酸型Monacolin K提取效果的影响,确定提取工艺中各因素最佳水平。结果红曲中酸型Monacolin K的最佳提取工艺:提取溶剂:70%的乙醇(pH调为7.5)、物料比1∶30、提取时间1.5h、提取温度50℃。结论该优选工艺稳定、可行,为提取红曲中酸型Monacolin K的工艺放大研究提供了基础数据。

OBJECTIVE To optimize the extraction process of acid type Monacolin K from Monascus.METHODS Single factor experiment was used to investigate the factors influencing the extraction process of acid type Monacolin K.Taking the extraction amount of acid type Monacolin K and the ratio of acid type Monacolin K to two forms of Monacolin K(acid type and lactone type)as the evaluation index,the influences of extraction solvent pH value,extraction solvent,material liquid ratio,extraction time,extraction temperature and other factors on the extraction effect of acid type Monacolin K were investigated,and the best level of each factor in the extraction process was determined.RESULTS The best extraction process for preparing acid type Monacolin K was as follows:extraction solvent:70%ethanol(pH adjusted to 7.5),material ratio:1∶30,extraction time:1.5 hours,extraction temperature:50℃.CONCLUSION The optimization process is stable and feasible,and it can provide basic data for the scale-up study of extracting acid Monacolin K from Monascus.