超高效液相色谱-质谱法检测养心定悸膏中阿胶及牛皮源成分

UPLC-MS Analysis of Donkey-hide Gelatin and Cowhide Source in YangXin DingJi Ointment

目的建立养心定悸膏中阿胶及掺杂牛皮源成分的专属性检测方法。方法利用胰蛋白酶对养心定悸膏中阿胶进行酶解,采用超高效液相色谱-质谱,以阿胶特征分子离子峰m/z 540.0(双电荷)→612.2、m/z 540.0(双电荷)→924.4及牛皮源特征离子对m/z 641.3(双电荷)→726.2、m/z 641.3(双电荷)→783.3作为检测离子对,离子化模式为ESI+,进行多反应监测。结果 7批养心定悸膏样品均检出了阿胶,且均未检出牛皮源成分。结论建立的检查方法准确、可靠、专属性强,可用于养心定悸膏中阿胶及掺杂牛皮源成分的检测。

OBJECTIVE To establish an analytical method to identify donkey-hide gelatin and cowhide source in YangXin DingJi Ointment.METHODS Identification of donkey-hide gelatin and cowhide source in YangXin DingJi Ointment was performed by rapid resolution liquid chromatography(UPLC)coupled to mass spectrometry(MS).The characteristic ion pairs of donkey-hide gelatin m/z 540.0→612.2 and m/z 540.0→924.4 were as detection ion pairs.The characteristic ion pairs of cowhide source m/z 641.3→726.2 and m/z 641.3→783.3 were as detection ion pairs.The positive electrospray ionization and multiple reaction monitoring were adopted.RESULTS Donkey-hide gelatin was detected in 7 batches samples,and cowhide source was not detected at the same time.CONCLUSION The methods were highly accurate,specific and reliable,which can be applied to detect the use of donkey-hide gelatin and cowhide in YangXin DingJi Ointment.