忽地笑不同器官RNA提取方法比较研究

Comparative Study on RNA Extraction Methods in Different Organs of Lycoris aurea Herb

[目的]建立忽地笑不同器官总RNA最佳提取方法。[方法]以忽地笑鳞茎、叶、花葶和花器官为材料,分别用普通试剂盒法、多糖多酚试剂盒法、Trizol法、SDS法和CTAB法进行不同器官总RNA提取方法比较。[结果]忽地笑不同器官最佳RNA提取方法不同,仅Trizol法能提取出鳞茎中RNA,普通试剂盒法和CTAB法均能提取出叶和花器官高质量RNA,多糖多酚试剂盒法和CTAB法能提取出花葶高质量RNA。[结论]普通试剂盒法是忽地笑叶和花的最快速、最简便的提取方法;多糖多酚试剂盒法是提取花葶的最佳提取方法;CTAB法能有效去除多糖、多酚,适合忽地笑叶、花葶和花中总RNA提取;试剂盒法和CTAB法均能提取高质量RNA,满足RT-PCR及后续试验的要求。

[Objective]To establish the best extraction method of total RNA from different organs of Lycoris aurea.[Methods]The methods of extraction of total RNA from bulb,leaf,calyx and flower different organs were compared by means of common reagent box method,polysaccharide polyphenol reagent box method,Trizol method,SDS method and CTAB method.[Result]The best RNA extraction method for different organs was different.Only Trizol method can extract RNA from bulbs,and both normal reagent box method and CTAB method could extract high-quality RNA from leaves and flower organs.Polysaccharide polyphenol reagent boxed method and CTAB method could extract high-quality RNA from calyx.[Conclusion]The common reagent box method is the fastest and simplest method for extraction of Lycoris aurea leaves and flowers.The polysaccharide polyphenol reagent box method is the best method for extraction of calyx.The CTAB method can effectively remove polysaccharides and polyphenols,which is suitable for the extraction of total RNA in Lycoris aurea leaves,calyx and flowers.The reagent box method and CTAB method can extract high-quality RNA to meet the requirements of RT-PCR and subsequent tests.