不同病原性相关分子模式对大黄鱼头肾巨噬细胞免疫功能的影响

Effects of Pathogen-associated Molecular Patterns on the Immunologic Function of the Head Kidney Macrophages from Large Yellow Croaker(Larimichthys crocea)

为研究不同病原性相关分子模式(PAMP)对大黄鱼头肾巨噬细胞免疫功能的影响,本研究分别将脂多糖(lipopolysaccharide,LPS)、肽聚糖(peptidoglycan,PGN)、脂磷壁酸(lipoteichoic acid,LTA)、胞壁酰二肽(muramyl dipeptide,MDP)与提取的大黄鱼头肾巨噬细胞进行孵育,测定大黄鱼头肾巨噬细胞的呼吸爆发活性、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、溶菌酶(LZM)活性和免疫相关细胞炎症因子(TNFα,IL1β,IL8,IL10,CXCL9,MYD88,TLR3和TLR8)的表达情况。结果显示:(1)LPS,PGN,LTA,MDP均能显著提高大黄鱼头肾巨噬细胞的呼吸爆发活性和LZM活性,同时显著降低SOD和CAT两种抗氧化酶的活性;(2)在LPS和LTA刺激下,8种免疫相关细胞炎症因子TNFα,IL1β,IL8,IL10,CXCL9,MYD88,TLR3和TLR8均呈显著性上调趋势;在PGN和MDP刺激下,除IL8,CXCL9外,其余6种免疫相关细胞炎症因子均显著性上调。结果表明,4种病原相关分子模式LPS,PGN,LTA,MDP均能显著影响大黄鱼头肾巨噬细胞的非特异性免疫应答能力和免疫相关细胞炎症因子的表达。

In order to study the effects of pathogen-associated molecular patterns on the immunologic function of the head kidney macrophages(HKMs)from large yellow croaker(Larimichthys crocea),the head kidney macrophages from L.crocea were extracted and then incubated with lipopolysaccharide(LPS),peptidoglycan(PGN),lipoteichoic acid(LTA),muramyl dipeptide(MDP),respectively,and their respiratory burst activity,antioxidant enzyme(SOD&CAT)activity,lysozyme activity and expression of immune-related cellular inflammatory factors(TNFα,IL1β,IL8,IL10,CXCL9,MYD88,TLR3,and TLR8)were detected.The results showed that the LPS,PGN,LTA,and MDP increased the respiratory burst activity and lysozyme activity of HKMs from L.crocea,but reduced the activities of the two antioxidant enzymes SOD and CAT.The expression levels of TNFα,IL1β,IL8,IL10,CXCL9,MYD88,TLR3,and TLR8 were significantly upregulated under the LPS and LTA stimulation,and other six immune-related cellular inflammatory factors except IL8 and CXCL9 were significantly up-regulated under the PGN and MDP stimulation.These results suggested that the four pathogen-associated molecular patterns,LPS,PGN,LTA and MDP,have significant effect on the non-specific immunity and the expression of immune-related cellular inflammatory factors of HKMs from L.crocea.