转玉米PEPC和PPDK基因对‘南林895杨’叶绿素参数影响的比较

Comparative of the Effects of Transgenic Maize PEPC and PPDK Genes on Chlorophyll Parameters of'Nanlin895'Poplar

磷酸烯醇式丙酮酸羧化酶(PEPC)和磷酸丙酮酸二激酶(PPDK)是C_4植物维持高效光合作用的关键酶,本研究通过农杆菌转化手段将玉米PEPC基因和PPDK基因成功导入‘南林895杨’,并进行叶绿素含量和荧光参数检测,结果显示:高光强胁迫下,所有转基因植株的叶绿素含量的下降幅度(34.2%~38.1%)均小于对照组(41.2%);转基因株系原初光化学效率下降幅度(29.0%~47.3%)和实际光化学效率下降幅度(29.5%~45.9%)均分别小于对照组(54.9%, 51.2%),二者具有一致性;高光照条件下,转基因株系光化学淬灭系数qP值(0.45~0.49)全面高于对照组(0.43);非光化学淬灭系数qN值显示转基因株系在高光照条件下与对照组相比没有明显的耐光氧化能力的提高。研究结果表明,转玉米PEPC和PPDK基因‘南林895杨’对于强光的利用能力增强,从而提高了转基因植株的光合性能。

Phosphoenol pyruvate carboxylase(PEPC)and phosphopyruvate dikinase(PPDK)are key enzymes for maintaining high photosynthesis efficiency in C4 plants.In this study,PEPC and PPDK genes from maize were successfully introduced into’Nanlin895’poplars by agrobacterium transformation,and chlorophyll content and fluorescence parameters were detected.Under high light stress,the decreases of chlorophyll content in all transgenic plants(34.2%~38.1%)were less than those in the control group(41.2%);the decreases of original photochemical efficiency(29.0%~47.3%)and actual photochemical efficiency(29.5%~45.9%)of the transgenic plants were less than those in the control group(54.9%,51.2%),respectively.The photochemistry quenching coefficient qP value(0.45~0.49)of the transgenic lines was higher than that of the control group(0.43)under high illumination,and the non-photochemistry quenching coefficient qN value showed that the photooxidation resistance of the transgenic lines was not improved significantly under high illumination compared with that of the control group.The results indicated that because the utilization of strong light of transgenic’Nanlin 895’poplars with PEPC and PPDK gene from maize were enhanced,the photosynthetic performance of transgenic plants was improved.