甘蔗新品种桂柳05136组培快繁试验

Tissue culture and rapid propagation of a new sugarcane variety Guiliu 05136

以甘蔗新品种桂柳05136的幼嫩叶片作为外植体,采用不同浓度2,4-D对幼嫩叶片的切片进行愈伤诱导,以不同浓度6-BA对愈伤组织进行分化诱导和增殖培养,以不同浓度NAA诱导组培苗生根。结果表明:适宜甘蔗幼嫩叶片愈伤诱导的培养基为改良MS+2 mg/L2,4-D,诱导分化培养以改良MS+2 mg/L6-BA为宜,增殖培养以改良MS+3 mg/L6-BA为宜,适宜的生根培养基为改良1/2MS+5 mg/L NAA。

Experiments were carried out using stem of sugarcane variety Guiliu 05136 as materials,the best synthetic way of tissure culture was obtained by different exogenous hormones ratio for inducing dedifferentiation and redifferentiation of callus,inducing germination of shoot-tip and axillary bud,clustered buds cultivation,multiplication during subculture and rooting.The results showed that for Guiliu 05136,modified MS+2 mg/L 2,4-D was the optimal for callus induction,modified MS+2 mg/L 6-BA was the optimal medium for buds differentiation from callus induction,modified MS+3 mg/L 6-BA was the optimal proliferation medium,and the best medium for rooting was modified 1/2MS+5 mg/L NAA.