摘要
以甘蔗新品种桂柳05136的幼嫩叶片作为外植体,采用不同浓度2,4-D对幼嫩叶片的切片进行愈伤诱导,以不同浓度6-BA对愈伤组织进行分化诱导和增殖培养,以不同浓度NAA诱导组培苗生根。结果表明:适宜甘蔗幼嫩叶片愈伤诱导的培养基为改良MS+2 mg/L2,4-D,诱导分化培养以改良MS+2 mg/L6-BA为宜,增殖培养以改良MS+3 mg/L6-BA为宜,适宜的生根培养基为改良1/2MS+5 mg/L NAA。
Experiments were carried out using stem of sugarcane variety Guiliu 05136 as materials,the best synthetic way of tissure culture was obtained by different exogenous hormones ratio for inducing dedifferentiation and redifferentiation of callus,inducing germination of shoot-tip and axillary bud,clustered buds cultivation,multiplication during subculture and rooting.The results showed that for Guiliu 05136,modified MS+2 mg/L 2,4-D was the optimal for callus induction,modified MS+2 mg/L 6-BA was the optimal medium for buds differentiation from callus induction,modified MS+3 mg/L 6-BA was the optimal proliferation medium,and the best medium for rooting was modified 1/2MS+5 mg/L NAA.
作者
韦海球
罗晟昇
唐利球
江清梅
Wei Hai-qiu;Luo Sheng-sheng;Tang Li-qiu;Jiang Qing-mei(Guangxi South Subtropical Agricultural Science Research Institute/Longzhou Tropical Crops Scientific Observation Station of Ministry of Agriculture,Chongzuo Guangxi 532415,China)
出处
《广西糖业》
2020年第4期26-28,共3页
Guangxi Sugar Industry
关键词
甘蔗
桂柳05136
组培快繁
sugarcane
Guiliu 05136
tissue culture and rapid propagation