PHLPP1在喉鳞癌中的表达及其与临床病理特征的相关性分析

Analysis on expression of PHLPP1 in laryngeal squamous cell carcinoma and the correlation with clinical pathological features

目的探讨PHLPP1在喉鳞癌转移过程中的表达情况及PHLPP1的表达与喉鳞癌患者临床病理特征的相关性,为喉鳞癌的临床治疗提供科学依据。方法收集2016年1月至2017年6月在台州医院耳鼻咽喉科手术切除并被病理科诊断证实为喉鳞癌的76例患者的病理资料,并筛选出其中发生癌转移的17例病例作为转移组,未发生转移的59例病例作为未转移组,同时纳入20例患者的正常喉黏膜作为对照组。比较各组PHLPP1的表达情况,并分析PHLPP1的表达与喉鳞癌患者临床病理特征的相关性,随访2年。采用SPSS 20.0统计软件进行χ^2检验或Fisher精确检验,采用KaplanMeier生存分析方法对喉鳞癌患者进行生存分析。结果 PHLPP1蛋白的表达主要定位于细胞质,偶有核表达,呈黄色或棕黄色颗粒。PHLPP1蛋白在喉鳞癌组组织中的阳性表达率(34/76,44.74%)明显低于对照组(20/20,100%),差异有统计学意义(P<0.01);PHLPP1蛋白在转移组组织中的阳性表达率(4/17,23.52%)明显低于未转移组(30/59,50.85%),差异有统计学意义(P<0.05)。PHLPP1蛋白表达水平在临床分期Ⅲ~Ⅳ期及发生淋巴结转移的患者组织中表达明显降低,差异均有统计学意义(P<0.01)。PHLPP1蛋白表达水平在不同性别、年龄、肿瘤发生部位、组织分化程度、是否脉管侵犯及是否发生远端转移患者组织中的表达差异均无统计学意义(P>0.05)。PHLPP1阳性组患者的2年生存率明显低于阴性组,差异有统计学意义(P<0.05)。结论 PHLPP1在喉鳞癌组织中表达显著下调,且与临床分期及是否发生淋巴结转移密切相关,提示PHLPP1可能在喉鳞癌的发生发展及转移过程中发挥重要的调控作用。

Objective To explore the expression of PHLPP1 in laryngeal squamous cell carcinoma(LSCC) metastasis and the correlation between the expression of PHLPP1 and clinical pathological features of LSCC patients,and to provide the scientific basis for clinical treatment of laryngeal squamous cell carcinoma. Methods From January of 2016 to June of 2017,76 inpatients with laryngeal squamous cell carcinoma diagnosed pathologically after operation in the ear-nose-throat department of Zhejiang Taizhou Hospital were collected,17 cases with metastasis and 59 cases without metastasis served as metastasis group and without metastasis group respectively,normal laryngeal mucosa of 20 cases served as the control group. The expression of PHLPP1 was compared among three groups,and the correlation between PHLPP1 expression and clinical pathological characteristics in patients was analyzed and was followed up for 2 years. The χ^2 test and Fisher test were used to analyze the data,Kaplan-Meier survival analysis was used to analyze the survival of patients with laryngeal squamous cell carcinoma. The used software was SPSS 20.0.Results The expression of PHLPP1 protein was mainly localized in cytoplasm,occasionally expressed in nucleus,with yellow or brown granules. The positive expression rate of PHLPP1 protein in laryngeal squamous cell carcinoma(34/76,44.74%) was significantly lower than that(20/20,100%) in normal control group,P<0.01. The positive expression rate of PHLPP1 protein in metastasis group(4/17,23.52%) was significantly lower than that(30/59,50.85%) in non-metastasis group,P <0.05. The expression level of PHLPP1 protein in clinical stage Ⅲ-Ⅳ and lymph node metastasis patients significantly decreased,P<0.01.But the expression of PHLPP1 protein was not significantly different in gender,age,location of tumors,degree of tissue differentiation,whether vascular invasion or distal metastasis occurred,P >0.05. The two-year survival rate of the laryngeal squamous cell carcinoma patients with PHLPP1 positive expression was significantly lower than that of the PHLPP1 negative expression group,P >0.05. Conclusion The expression of PHLPP1 in laryngeal squamous cell carcinoma significantly down-regulated,which was closely related to clinical stage and lymph node metastasis,suggesting that PHLPP1 may play an important regulatory role in the occurrence,development and metastasis of laryngeal squamous cell carcinoma.