大鼠颈总动脉球囊拉伤模型的制作研究

Establishment of Animal Model of Balloon Injury in Rat Carotid Artery

目的通过对大鼠颈总动脉球囊拉伤模型制作技术的创新和探索,不断优化策略提高动物模型的成活率和成材率,形成稳定的造模技术,为经皮冠状动脉腔内成形术术后并发症的预防和治疗提供实验基础。方法将25只Sprague-Dawley(SD)大鼠随机分为三组,分别为大球囊组(球囊直径2.0 mm)11只、小球囊组(球囊直径1.5 mm)11只、假手术组3只,大球囊组和小球囊组应用不同大小的球囊进行模型制作,术后14 d通过伊凡氏蓝染色进行验证,动脉拉伤后颈总动脉内膜成功着色者为造模成功。结果小球囊组造模成功10例(成功率90.9%,10/11),大球囊组造模成功5例(成功率45.5%,5/11),两组造模成功率差异具有统计学意义,两组内膜损伤面积无显著差异。结论应用1.5 mm球囊可以实现对内膜的有效损伤,且造模成功率较2.0 mm球囊显著升高。

Objective To optimize the establishment of rat carotid artery balloon-injured model and to improve success rate in it.Method Twenty-five Sprague-Dawley(SD)rats were randomly divided into three groups.The rats’left carotid artery in group A were deendothelialized by passing an inflatable balloon catheter(size 2 mm,Cordis).The rats’left carotid artery in group B were deendothelialized by passing an inflatable balloon catheter(size 1.5 mm,Cordis).The rats’left carotid artery in group C were made an arteriotomy without passing balloon catheter.The effect of balloon injury was assessed via Ivans’blue staining.Result The success rate in establishment of rat carotid artery balloon-injured model of group A is 45.5%(5/11).The success rate of group B is 90.9%(10/11)(P=0.032).The difference of injured carotid artery intima area between group A and B is not significant(P=0.426).Conclusion The application of 1.5 mm inflatable balloon catheter could successfully denude the intima of carotid artery in rats and could significantly improve the successful establish rate of rat carotid artery balloon-injured model.