遗传工程小鼠冻存卵巢异体原位移植比较研究

Study for Cryopreservation and Orthotopic Transplantation of Genetically Engineering Mice Ovary

目的对C57BL/6 J为背景的遗传工程小鼠卵巢冷冻及复苏后原位移植进行研究,建立遗传工程小鼠卵巢冷冻方法,弥补小鼠资源保种体系。方法采用DAP213方法冷冻保存了C57BL/6 J及SCARB2、PSGL1、IGB3S三个遗传工程小鼠10日龄幼鼠的卵巢,将四个品系10日龄幼鼠的新鲜卵巢及冻存复苏卵巢原位移植到4周龄C57BL/6 J及C57BL/6 J(Cg)—Tyr^c-2 J/J(B6 albino)受体雌鼠,术后饲养5周与10周龄性成熟C57BL/6 J雄鼠交配,观察移植出生幼仔。结果C57BL/6 J新鲜卵巢和冻存卵巢,三个品系遗传工程小鼠的冻存卵巢原位移植到C57BL/6 J和B6 albino受体中,均可以恢复正常功能,交配后得到仔鼠。结论采用DAP213方法冻存C57BL/6 J及以C57BL/6 J为背景的遗传工程小鼠卵巢,复苏后原位移植到C57BL/6 J和B6 albino,交配后产仔,卵巢冻存方式是小鼠资源库保存的一个重要补充。

Objective To explore a method of cryopreservation and orthotopic transplantation of genetically engineering mice ovary of C57BL/6J background,establish a cryopreservation system.Method The ovaries from 10 d of C57BL/6J and three genetically engineering mice were freezed with DAP21310 d fresh ovaries and thawed ovaries were grafed to the 4 weeks C57BL/6J and C57BL/6J(Cg)—Tyr^c-2 J/J(B6 albino)recipient,then mated with 10 weeks C57BL/6J male mice after 5 weeks breeding,observed offspring.Result The grafted C57BL/6J fresh ovaries and freeze-thawed ovaries and genetically engineering mice freeze-thawed ovaries could be restored reproduction,and gotten normal pups when mated with the same strain males.Conclusion Cryopreservation of C57BL/6J and genetically engineering mice ovaries with DAP213,were thawed and grafted to recipient,then mated with male,gotten normal pups.Cryopreservation of ovaries is a important method for mice resource bank.