SPRED1在结肠癌中的表达及意义

Expression of SPRED1 in Colon Cancer and Its Significance

[目的]探讨SPRED1在结肠癌中的表达,及其与结肠癌患者预后的相关性,以及对结肠癌细胞增殖、迁移和凋亡的影响。[方法]采用实时荧光定量聚合酶链反应(qRT-PCR)检测SPRED1 m RNA在结肠癌细胞和组织标本中的表达,免疫组织化学技术检测SPRED1蛋白在结肠癌组织中的表达。使用小干扰RNA(siRNA)干扰结肠癌细胞内SPRED1表达后,CCK-8试剂盒检测结肠癌细胞增殖能力变化,Transwell小室检测结肠癌细胞迁移能力变化,Annexin V-FITC/PI凋亡试剂盒检测细胞凋亡水平。[结果] SPRED1 mRNA在结肠癌细胞株和结肠癌组织中表达水平均显著上调(P均<0.001),高表达SPRED1患者的总生存期短于低表达SPRED1患者(HR=2.326,95%CI:1.340~4.062,P<0.05)。干扰SPRED1后,干扰组结肠癌SW480和SW620细胞在36h和48h后增殖能力显著下降(P<0.05),侵袭能力显著下降(SW480:t=42.05,P<0.0001;SW620:t=12.91,P=0.0002),且凋亡水平显著上升(SW480:t=18.46,P<0.05;SW620:t=18.52,P<0.0001)。[结论]SPRED1在结肠癌中表达上调,促进结肠癌的增殖和侵袭。

[Objective] To investigate the expression of SPRED1 in colon cancer and its significance.[Methods] The expression of SPRED1 m RNA and protein in cancer tissue and adjacent tissue samples of 45 patients with colon cancer was detected with q RT-PCR and immunohistochemistry,respectively. The expression of SPRED1 m RNA and protein was detected in colon cancer SW480,SW620 cells and normal colon epithelial NCM460 cells with Real-time quantitative RT-PCR(q RTPCR) and Western blot,respectively. The colon cancer SW480 and SW620 cells were transfected with small interference RNA(si-SPRED1). After down-regulation of SPRED1,cell proliferation,cell migration and cell apoptosis were determined by CCK8 assay,Transwell method and Annexin VFITC/PI assay,respectively. [Results] The expression of SPRED1 m RNA and protein was higher in colon cancer tissues than that in adjacent tissues,in cancer cell lines than in normal colon epithelia cells(all P<0.001).The overall survival time of patients with high expression of SPRED1 was shorter than that of patients with low expression of SPRED1(HR=2.326,95%CI:1.340~4.062,P<0.05). After down-regulation of SPRED1,cell proliferation was significantly suppressed(P<0.05).Both SW480 and SW620 cells presented decreased cell migration after down-regulation of SPRED1(SW480:t=42.05,P<0.0001;SW620:t=12.91,P=0.0002). colon cancer Cells transfected with si-SPRED1 showed higher apoptosis level(SW480:t =18.46,P <0.05;SW620:t =18.52,P <0.0001). [Conclusion] SPRED1 is upregulated in colon cancer,which is associated with the increased cell proliferation,cell invasion and decreased cell apoptosis.