木薯HSP21基因的电子克隆与生物信息学分析

In silico cloning and bioinformatics analysis of HSP21 in Manihot esculenta

HSP21是植物响应高温胁迫的关键基因,在防止蛋白变性、保护细胞结构和维持植物正常生长发育等方面发挥重要作用,克隆HSP21基因是揭示木薯抵抗高温胁迫分子机制的基础。为得到木薯HSP21同源基因及分析其表达蛋白的性质,文中采用电子克隆技术对新基因进行组装和衍生,并使用生物信息学分析方法,对预测蛋白的一级至高级结构、亲水性/疏水性、信号肽、蛋白同源性和系统进化等进行全面解析。结果表明,HSP21基因含有969个碱基对,其开放阅读框有705个碱基,预测蛋白含234个氨基酸。预测蛋白是非跨膜蛋白,具有碱性和亲水性,主要定位于叶绿体内。通过多重序列比对和系统进化分析发现,木薯与巴西橡胶树、蓖麻和麻疯树等植物的HSP21蛋白同源性较高。结果可为该基因的克隆和转化提供参考依据。

HSP21 gene is a key gene to respond high temperature stress in plant and plays an important role in preventing protein denaturation,protecting cell structure and maintaining normal growth and development.Therefore,cloning HSP21 gene is the basis for revealing the molecular mechanism of resistance to high temperature stress in cassava.To obtain cassava HSP21 homologous gene and analyze the properties of predicted protein,electronic cloning technology was used to assemble and derivate new gene in this study,and bioinformatics analysis method was used to analyze the primary to highest structure,hydrophilicity/hydrophobicity,signal peptide,protein homology and phylogenetic evolution of expressed protein.HSP21 gene was 969 bp,its open reading frame was 705 bp,and the predicted protein contains 234 amino acids.The predicted protein is a non-transmembrane protein that is alkaline and hydrophilic,and is mainly localized in the chloroplast.Through multiple sequence alignment and phylogenetic analysis,it was found that the cassava HSP21 protein has high homology with other plants such as Hevea brasiliensis,Ricinus communis,and Jatropha curcas.The results could provide reference for the study of cloning and transformation of this gene.