摘要
目的探讨晚期氧化蛋白产物(AOPP)对肝细胞凋亡的影响及其相关机制。方法体外制备AOPP处理体外培养的小鼠AML-12肝细胞,采用流式细胞仪检测细胞凋亡比例及细胞内活性氧(ROS)的变化,免疫组织化学法染色(Western blotting)检测凋亡蛋白3(caspase 3)以及裂解凋亡蛋白3(cleaved caspase 3)的表达水平。结果AOPP处理呈浓度依赖性激活caspase 3以及增加AML-12细胞凋亡数量;AOPP刺激可显著促进细胞内ROS生成,ROS清除剂预处理可缓解AOPP诱导细胞内ROS生成、caspase 3激活及肝细胞凋亡比例。结论AOPP通过ROS依赖性激活caspase 3途径,进而诱导肝细胞发生凋亡。
Objective The study aims to explore the mechanism and impact of advanced oxidation protein products(AOPP)on hepatocyte apoptosis.Methods Rat liver cells(AμG-12)was disposed with AOPP in vitro.The effects of AOPP on hepatocyte apoptosis were evaluated by measuring apoptosis ratio,intracellular reactive oxygen species(ROS)through flow cytometry and expression of caspase 3,cleaved caspase 3 through Western blotting.Results AOPP treatment significantly up-regulated the expression of caspase 3 and ROS as well as AμG-12 apoptosis with dose-dependent.Pretreatment of ROS scavenger could reverse the AOPP-induced caspase 3,ROS and AμG-12 apoptosis increase.Conclusion AOPP induce hepatocyte apoptosis through ROSdependent activation of caspase 3.
作者
孙世波
谢芳
彭丽珊
冯伟标
梁可一
周杰
SUN Shi-bo;XIE Fang;PENG Li-shan;FENG Wei-biao;LIANG Ke-yi;ZHOU Jie(Department of Hepatobiliary Surgery,Nanfang Hospital,Southern Medical University,Guangzhou,Guangdong 510515,China;Guangdong Provincial Key Laboratory of Gastroenterology,Department of Gastroenterology,Nanfang Hospital,Southern Medical University,Guangzhou,Guangdong 510515,China Southern;Medical University,Guangzhou,Guangdong 510515,China)
出处
《现代消化及介入诊疗》
2020年第8期1046-1050,1054,共6页
Modern Interventional Diagnosis and Treatment in Gastroenterology
基金
国家自然科学基金青年项目(81800454)
广东省自然科学基金(2018A030313219)
广东省医学科研基金(A2018049)。
关键词
晚期氧化蛋白产物
活性氧
凋亡蛋白3
肝细胞
Advanced oxidation protein products
Reactive oxygen species
Caspase 3
Liver cells
