摘要
为筛选优化褐黄木耳母种培养基配方,以菌丝日均长速为测量指标,菌丝长势为参考项,通过单因素试验,筛选出母种培养基最佳碳源和最佳氮源,并运用正交试验法优化配方。结果表明,褐黄木耳母种培养基最佳配方为蔗糖2%,酵母浸膏0.5%,KH2PO40.3%,MgSO4·7H2O 0.15%,琼脂2%,pH自然。利用该配方培养,褐黄木耳菌丝色白、密实,平均生长速度3.82 mm/d,高于正交试验和单因素试验结果。
Taking the average daily growth rate of hypha as the measurement index and hypha growth potential as the reference item,the best carbon source and the best nitrogen source of Auricularia fuslosuccinea stock culture medium were selected through single factor experiment,and the formula of stock culture medium was optimized by orthogonal test.The results showed that the optimal formula of stock culture medium for Auricularia fuslosuccinea was:sucrose 2%,yeast extract 0.5%,KH2PO40.3%,MgSO4·7H2O 0.15%,agar 2%,and natural pH.
作者
聂建军
潘保华
李彩萍
牛宇
徐全飞
冯婉君
蒙秋霞
Nie Jianjun;Pan Baohua;Li Caiping;Niu Yu;Xu Quanfei;Feng Wanjun;Meng Qiuxia(Institute of Agricultural Resources&Economy,Shanxi Academy of Agricultural Sciences,Taiyuan,Shanxi 030006,China;Institute of Agricultural Environment&Resources,Shanxi Academy of Agricultural Sciences,Taiyuan,Shanxi 030006,China)
出处
《食药用菌》
2020年第5期318-320,327,共4页
Edible and Medicinal Mushrooms
基金
山西省农业科学院山西“农谷”研发专项资助项目(YCX2018204)
山西省农业科学院农业科技成果转化与示范推广资助项目(2019CGZH07-2)
山西省重点研发计划(农业)资助项目(201803D221011-1)
山西省重点研发计划资助项目(201903D221046)。
关键词
褐黄木耳
正交试验
母种培养基
配方
优化
Auricularia fuslosuccinea
orthogonal test
stock culture medium
formula
optimization
