摘要
研究大孔树脂对油菜叶多糖的纯化工艺,并就纯化后的多糖对DPPH、ABTS、羟基自由基的清除作用进行考察。结果表明:AB-8型大孔树脂为最佳纯化树脂;最佳吸附条件为pH4.0、上样浓度6.0 mg/mL、上样速率1.0 BV/h;最佳解吸条件为乙醇体积分数70%、洗脱剂用量3.0BV、洗脱速率2.0BV/h。纯化后的油菜叶多糖含量从9.84%提高到59.56%,对几种自由基都有很好清除作用,且随着多糖浓度增大而增强。
The purification process of polysaccharides from leaves of Brassica napus L.was studied,and the scavenging effects of the purified polysaccharides on DPPH,ABTS and hydroxyl radicals were investigated.The results showed that AB-8 was the best resin for the purification of polysaccharides.The best dynamic adsorption conditions were:pH 4.0,the concentration of sample 6.0 mg/mL,the sample loading speed 1.0 BV/h.The best dynamic desorption conditions were:the ethanol concentration 70%,the amount of eluent 3.0 BV,the flow rate 2.0 BV/h.The content of the purified polysaccharide increased from 9.84%to 59.56%,which had a good scavenging effect on several free radicals and increased with the increase of the increase of the concentration of polysaccharide.
作者
叶润
蔡静
赵丽平
乔新荣
YE Run;CAI Jing;ZHAO Li-ping;QIAO Xin-rong(School of Biology and Pharmaceutical Engineering,Xinyang Agriculture and Forestry University,Xinyang 464000,Henan,China)
出处
《粮食与油脂》
北大核心
2020年第9期102-106,共5页
Cereals & Oils
基金
河南省自然科学基金项目(162300410227)
河南省科技攻关项目(172102310425)
河南省科技发展计划项目(162102210364)
信阳市社会哲学类项目(2019JJ052)
校青年基金项目(2018LG012、2019LG001)。
关键词
油菜叶
多糖
大孔树脂
抗氧化活性
leaf of Brassica napus L.
polysaccharide
macroporous resin
antioxidant activity