β-Tubulin-Ⅲ在肺腺癌术前分期诊断中的应用分析

Analysis of β-Tubulin-Ⅲ application in diagnosis of lung adenocarcinoma during preoperative stage

目的探讨β-Tubulin-Ⅲ在肺腺癌术前分期诊断中的应用价值。方法收集拟行手术治疗的60例肺腺癌患者的病历资料,于术前分别采用免疫组织化学染色法、光学显微镜法检测血清β-Tubulin-Ⅲ表达水平,结合影像、随访结果,分析其在术前分期诊断中的价值。结果不典型腺瘤样增生(AAH)、原位癌(AIS)、微浸润性腺癌(MIA)肺腺癌组织中均基本无β-Tubulin-Ⅲ表达,浸润性腺癌(IA)肺腺癌组织中β-Tubulin-Ⅲ表达情况均优于AAH、AIS及MIA,差异均有统计学意义(P﹤0.05)。T3~4期肺腺癌患者β-Tubulin-Ⅲ表达量高于T1~2期患者,差异有统计学意义(P﹤0.05);N0~1期肺腺癌患者无β-Tubulin-Ⅲ阳性表达,N3期肺腺癌患者β-Tubulin-Ⅲ表达量高于N2期患者,差异有统计学意义(P﹤0.05)。前沿区、非前沿区分化情况比较,差异有统计学意义(P﹤0.05)。结论β-Tubulin-Ⅲ强阳性表达可提示肺腺癌早期浸润,能够为手术治疗方案的制订提供参考。

Objective To investigate the application value of β-Tubulin-Ⅲ in the diagnosis of lung adenocarcinoma during preoperative stage.Method The medical data of 60 patients with lung adenocarcinoma who were to be treated with surgery regimen were collected.Serum β-Tubulin-Ⅲ was detected by immunohistochemical staining assay and optical microscopy method before surgery.The expression level combined with the results of imaging and follow-up,its value in preoperative staging diagnosis was analyzed.Result There was no expression of β-Tubulin-Ⅲ in atypical adenomatous hyperplasia(AAH),adenocarcinoma in situ(AIS),and minimally invasive adenocarcinoma(MIA),and the expression level of β-Tubulin-Ⅲ was more obvious in invasive adenocarcinoma(IA)tissues than that in the foregoing types and the differences were statistically significant(P<0.05).The expression of β-Tubulin-Ⅲ in patients with T3-4 lung adenocarcinoma was higher than that in T1-2,and the difference was statistically significant(P<0.05).There was no expression of β-Tubulin-Ⅲ in patients with N0-1 lung adenocarcinoma.The expression of β-Tubulin-Ⅲ in patients with N3 lung adenocarcinoma was higher than that in stage N2,and the difference was statistically significant(P<0.05).The difference between frontier area and non-frontier area is statistically significant(P<0.05).Conclusion The strong positive expression of β-Tubulin-Ⅲ may indicate the early infiltration of lung adenocarcinomaand can provide a reference for the establishment of surgical treatment regimen.