泛素连接酶PC2在鼻咽癌发生中的作用

The Role of Ubiquitin Ligase PC2 in the Development of Nasopharyngeal Carcinoma

目的:探究泛素连接酶PC2在鼻咽癌发生中的作用。方法:选择2017年3月至2018年8月于我院接受治疗的68例鼻咽癌患者为研究组,选择同期于我院接受治疗的50例鼻咽良性病变患者为良性对照组,选择同期于我院接受体格检查的50例健康个体为对照组,分别检测3组个体泛素连接酶PC2及其mRNA表达水平并实施组间对比,而后将鼻咽癌患者按照病情严重程度分为Ⅰ-Ⅱ期和Ⅲ-Ⅳ期两组,对比两组泛素连接酶PC2及其mRNA表达水平,最后对鼻咽癌患者实施为期12个月的随访,按照其结局区分为死亡组(20例)和存活组(48例),对比两组患者泛素连接酶PC2及其mRNA表达水平。结果:(1)研究组泛素连接酶PC2蛋白的表达量和mRNA表达水平均显著高于良性对照组,且良性对照组的上述指标也显著高于对照组(P<0.05);(2)Ⅲ-Ⅳ期患者泛素连接酶PC2蛋白的表达量及mRNA表达水平高于Ⅰ-Ⅱ期患者(P<0.05);(3)死亡组泛素连接酶PC2蛋白的表达量及mRNA表达水平高于存活组(P<0.05)。结论:泛素连接酶PC2的高表达与鼻咽癌病情具有一定相关性,可以作为鼻咽癌诊断及预后评估指标之一。

Objective:To explore the role of ubiquitin ligase PC2 in the development of nasopharyngeal carcinoma.Methods:68 NPC patients who were treated in our hospital from March 2017 to August 2018 were selected as the experimental group,50 NPC patients who were treated in our hospital at the same time were selected as the benign control group,and 50 healthy individuals who were examined in our hospital at the same time were selected as the control group.The expression levels of ubiquitin ligase PC2 and its m RNA in three groups were detected and compared between groups After that,NPC patients were divided into two groups according to the severity of the disease:Ⅰ-ⅡandⅢ-Ⅳ.the expression level of ubiquitin ligase PC2 and its mRNA were compared between the two groups.Finally,NPC patients were followed up for 12 months.According to the outcome,they were divided into death group(20 cases)and survival group(48 cases).The expression level of ubiquitin ligase PC2 and its mRNA were compared between the two groups.Results:(1)The expression level of ubiquitin ligase PC2 protein and m RNA expression level in the study group were significantly higher than those in the benign control group,and the above indicators in the benign control group were significantly higher than those in the control group(P<0.05).(2)The level of ubiquitin ligase PC2 protein and its m RNA expression in the III-IV patients were higher than that in the I-II patients(P<0.05).(3)The expression of ubiquitin ligase PC2 protein and m RNA in the death group was higher than that in the survival group(P<0.05).Conclusion:The high expression of ubiquitin ligase PC2 has a certain correlation with the state of NPC,which can be used as one of the indexes for the diagnosis and prognosis of NPC.