血管内皮祖细胞外泌体调控骨髓间充质干细胞基因表达谱芯片

Microarray Analysis of MSCs Gene Expression Regulated by Endothelial Progenitor Cells Derived from Exosomes

目的探讨兔外周血内皮祖细胞(endothelial progenitor cells derived from peripheral blood,EPCs)外泌体(Exosomes)调控骨髓间充质干细胞(bone marrow stromal cells,MSCs)差异基因表达情况。方法将EPC-Exos和MSCs共培养作为实验组,单独MSCs作为对照组,各培养14d后进行基因芯片送检,筛选出分子网络模块和关键基因。结果从高通量芯片数据中共筛选到差异基因表达共1772个,其中表达上调基因664个,下调基因1108个。基因表达差异主要与染色体、着丝粒等细胞器定位、细胞增殖、细胞周期等密切相关;EPC-exos调控MSCs显著富集的通路中P53通路、MARK通路及酮体合成和降解途径差异明显。结论利用基因芯片方法筛选的相关基因可能在EPC-Exos调控MSCs生物学功能中发挥重要作用,并可作为外泌体修复糖尿病创面的的理论依据。

Objective This study aimed to investigate the differential gene expression of rabbit bone marrow stromal cells(MSCs)through the regulation of endothelial progenitor cells derived from peripheral blood(EPCs)exosomes.Methods EPC-exos co-cultured with MSCs were used as the experimental group,and MSCs cultured separately were used as the control group.The gene chip was sent for detection to screen out the molecular network module and key genes after 14 days.Results 1772 differentially expressed genes were screened from high-throughput microarray data,of which 664 were up-regulated and 1108 were down-regulated.The differentially expressed genes were mainly related to the localization of organelles,the proliferation and cell cycle of organelles such as chromosomes and centromeres.In the pathways regulated by EPC-exos,the P53 pathway,the MARK pathway and the ketone synthesis and degradation pathway showed significant differences.Conclusion The relevant genes screened by gene chip method may play an important role in promoting the biological function of EPC-exos in regulating MSCs,and provide the theoretical basis for tissue and organ repair by exosomes.