EV-D68 VP1蛋白的生物信息学分析和候选疫苗表位肽段预测及筛选

Bioinformatics analysis of EV-D68 VP1 protein and prediction and screening of candidate vaccine epitope peptide segments

目的:通过对人肠道病毒D68型(EV-D68)衣壳蛋白VP1理化性质、结构功能和B细胞表位分析,获取候选疫苗肽段用于后期疫苗研制。方法:应用Bioedit软件、Netphos和ExPASy等在线工具分析EV-D68 VP1蛋白氨基酸序列。结果:EV-D68 VP1蛋白是等电点为8.73、相对分子量34.0 kD的亲水性蛋白质,具有35个可能的磷酸化位点,无信号肽和跨膜区,二级结构中不规则卷区有最高比例,α-螺旋和β-折叠散落在蛋白中;预测VP1蛋白具有多个可能的B细胞表位。结论:本研究分析了EV-D68 VP1的基本理化性质,结构功能和可能的B细胞表位,筛选了B细胞表位肽段,为EV-D68的进一步研究和疫苗的制备奠定基础。

Objective:By analyzing physicochemical properties,structural functions and B-cell epitopes of capsid protein VP1 of human enterovirus D68(EV-D68)to obtain peptide segments of candidate vaccine and use for later vaccine development.Methods:Amino acid sequence of EV-D68 VP1 was analyzed by Bioedit software,Netphos and ExPASy online tools.Results:EV-D68 VP1 was a hydrophilic protein with a isoelectric point of 8.73 and relative molecular weight of 34.0 kD.Protein containsed 35 phosphorylation sites and but no signal peptide and transmembrane domains.In the secondary structure,irregular curls were the most common,α-helix andβ-fold were scattered in VP1 protein.It was predicted that VP1 protein had many possible B-cell epitopes.Conclusion:Physicochemical properties,structure and function characteristics,and possible B-cell epitopes of EV-D68 VP1 were successfully predicted,and the best B-cell epitope peptide segments were screened out,which laid foundations for further study and the preparation of vaccines for EV-D68 infection.