摘要
目的:探讨人嗜中性粒细胞弹性蛋白酶(HNE)体外干预对嗜中性粒细胞型鼻息肉(NNP)上皮中杯状细胞(GC)合成和释放黏蛋白(MUC5AC)的影响。方法:收集正常鼻黏膜组织和NP组织,免疫荧光染色法鉴定鼻息肉(NP)内表型。将2种组织进行体外培养,HE染色检测GC数量,ELISA和RT-PCR法检测MUC5AC mRNA及蛋白表达。将HNE加入体外培养的NNP组织,进行GC计数,ELISA和RT-PCR法分别检测NNP培养基中MUC5AC mRNA表达。结果:与正常鼻黏膜组织相比,体外培养的NNP组织中GC数明显上升,MUC5AC mRNA及蛋白水平显著上升,当HNE对NNP组织进行体外干预后,GC数及MUC5AC mRNA及蛋白水平均显著上调。结论:HNE体外干预可促进NNP中MUC5AC的合成和释放。
Objective:To explore impact of human neutrophils elastase(HNE)intervention on production and release of MUC5AC from glolet cells GC in neutrophilic nasal polyps(NNP)in vitro.Methods:Normal nasal mucosa and NP tissues were selected.Immuofluorescent staining to identify endotype of Nosal polyps(NP).These two kinds of tissues were cultured in vitro.HE staining to examine numbers of GC,ELISA and RT-PCR to assess MUC5AC protein and mRNA expressing.HNE were then administered into NNP cultures,and GC numbers were counted.Protein and mRNA expressions of MUC5AC were evaluated by ELISA and real-time RT-PCR.Results:Compared with normal nasal mucosa,numbers of GC were increased in NNP.Expressions of MUC5AC protein and mRNA were also upregulated.After intervention of HNE,GC numbers and protein and mRNA levels of MUC5AC were increased significantly.Conclusion:HNE can promote production and release of MUC5AC from NNP in vitro.
作者
蔺林
戴飞
任国强
魏瑾瑾
陈峥
汤欣玥
LIN Lin;DAI Fei;REN Guo-Qiang;WEI Jin-Jin;CHEN Zheng;TANG Xin-Yue(Department of Otorhinolaryngology-Head and Neck Surgery,Huashan Hospital North of Fudan University,Shanghai 201907,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2020年第15期1873-1876,共4页
Chinese Journal of Immunology
基金
复旦大学附属华山医院北院启动基金(HSBY2019002)资助。