腺苷对肺癌细胞增殖的影响及其机制

Effect of adenosine on proliferation of lung cancer cells and its mechanism

目的研究腺苷对肺癌细胞增殖的作用,并探究其潜在分子机制。方法取对数生长期A549细胞分为空白对照组和0.5、1.0、2.5、5.0 mmol·L-1腺苷处理组,分别加入含0.0、0.5、1.0、2.5、5.0 mmol·L-1腺苷的培养液进行培养。采用细胞计数试剂盒-8检测腺苷对细胞增殖的影响,流式细胞术检测腺苷对A549细胞周期和凋亡的影响,划痕修复实验检测细胞迁移能力,实时荧光定量聚合酶链反应检测腺苷对A549细胞凋亡相关分子表达水平的影响。结果0.5、1.0、2.5、5.0 mmol·L-1腺苷处理组A549细胞培养24、48 h的生长抑制率均显著高于空白对照组(P<0.05);各浓度腺苷处理组A549细胞培养24、48 h的生长抑制率随浓度的升高逐渐增加,两两比较差异均有统计学意义(P<0.05)。2.5、5.0 mmol·L-1腺苷处理组A549细胞相对迁移率均显著低于空白对照组(P<0.05);5.0 mmol·L-1腺苷处理组A549细胞相对迁移率显著低于2.5 mmol·L-1腺苷处理组(P<0.05)。与空白对照组比较,2.5 mmol·L-1腺苷处理组A549细胞处于G 0/G 1期比例显著降低,S期比例升高,G 2/M期比例升高(P<0.05);5.0 mmol·L-1腺苷处理组A549细胞处于G 0/G 1期比例显著降低,G 2/M期比例显著升高(P<0.05);空白对照组和5.0 mmol·L-1腺苷处理组A549细胞处于S期比例比较差异无统计学意义(P>0.05)。与2.5 mmol·L-1腺苷处理组比较,5.0 mmol·L-1腺苷处理组A549细胞处于S期比例降低,G 2/M期比例升高(P<0.05);2.5 mmol·L-1腺苷处理组和5.0 mmol·L-1腺苷处理组细胞处于G 0/G 1期比例比较差异无统计学意义(P>0.05)。2.5、5.0 mmol·L-1腺苷处理组A549细胞凋亡率显著高于空白对照组(P<0.05);5.0 mmol·L-1腺苷处理组A549细胞凋亡率显著高于2.5 mmol·L-1腺苷处理组(P<0.05)。5.0 mmol·L-1腺苷处理组细胞中Bak、Caspase-3 mRNA相对表达量显著高于空白对照组(P<0.05);2组细胞中Bcl-2、Mcl-1 mRNA相对表达量比较差异无统计学意义(P>0.05)。结论腺苷可通过诱导A549细胞周期阻滞和凋亡来抑制细胞增殖能力,腺苷诱导A549细胞凋亡与其上调促凋亡因子Bak基因表达从而改变线粒体膜通透性有关。腺苷还可抑制A549细胞迁移能力。

Objective To investigate the effect of adenosine on proliferation of lung cancer cells and the potential molecular mechanism.Methods The A549 cells in logarithmic growth phase were taken and divided into the blank control group and 0.5,1.0,2.5,5.0 mmol·L-1 adenosine treatment group;the above five groups were added 0.0,0.5,1.0,2.5,5.0 mmol·L-1 adenosine medium for culture.The effect of adenosine on A549 cells proliferation was detected by cell counting kit-8,the cell cycle and cell apoptosis were examined by flow cytometry,the cells migration capacity was detected by scratch assay,the expression of apoptosis related molecular was detected by quantitative real-time polymerase chain reaction.Results The growth inhibition rate of A549 cells in the 0.5,1.0,2.5,5.0 mmol·L-1 adenosine treatment group was signi-ficantly higher than that in the blank control group at 24,48 h after treatment(P<0.05);the growth inhibition rate of A549 cells in different concentrations of adenosine treatment groups increased with the increase of the concentration of adenosine at 24,48 h after treatment,and the difference was statistically significant(P<0.05).The relative migration rates of A549 cells in 2.5 mmol·L-1 and 5.0 mmol·L-1 adenosine treatment groups were significantly lower than those in the blank control group(P<0.05);the relative migration rate of A549 cells in 5.0 mmol·L-1 adenosine treatment group was significantly lower than that in the 2.5 mmol·L-1 adenosine treatment group(P<0.05).Compared with the blank control group,the proportion of A549 cells in G 0/G 1 phase decreased and in S,G 2/M phase increased in the 2.5 mmol·L-1 adenosine treatment group(P<0.05);the proportion of A549 cells in G 0/G 1 phase decreased in G 2/M phase increased in the 5.0 mmol·L-1 adenosinetreatment group(P<0.05);there was no significant difference in the proportion of A549 cells in S phase between the blank control group and the 5.0 mmol·L-1 adenosine treatment group(P>0.05).Compared with the 2.5 mmol·L-1 adenosine treatment group,the proportion of A549 cells in S phase decreased,while that in G 2/M phase increased in the 5.0 mmol·L-1 adenosine treatment group(P<0.05);there was no significant difference in the proportion of A549cells in G 0/G 1 phase between the 2.5 mmol·L-1 adenosine treatment group and 5.0 mmol·L-1 adenosine treatment group(P>0.05).The apoptosis rates of A549 cells in the 2.5 mmol·L-1 and 5.0 mmol·L-1 adenosine treatment group were significantly higher than those in the blank control group(P<0.05);the apoptosis rate of A549 cells in the 5.0 mmol·L-1 adenosine treatment group was significantly higher than that in the 2.5 mmol·L-1 adenosine treatment group(P<0.05).The relative expression of Bak,Caspase-3 mRNA in A549 cells in the 5.0 mmol·L-1 adenosine treatment group was significantly higher than that in the blank control group(P<0.05);there was no significant difference in the relative expression of Bcl-2 and Mcl-2 mRNA in A549 cells between the two groups(P>0.05).Conclusions Adenosine can inhibit the proliferation of A549 cells by inducing cell apoptosis and cell cycles arrest.Adenosine induce the apoptosis of A549 cells was associated with the up-regulation of Bak gene expression.Adenosine also can inhibite the migration of A549 cells.