广东湛江竹节树自然种群遗传结构及遗传分化

Genetic Structure and Genetic Divergence in the Natural Population of Carallia brachiate From Zhanjiang of Guangdong Province

利用基因组SSR技术,研究广东湛江竹节树自然种群的遗传结构、遗传分化、基因流和小尺度空间遗传结构特征。结果表明:(1)竹节树种群遗传多样性较高,有效等位基因数Na=9.285、期望杂合度He=0.607、Shanoon′s多样性指数I=1.437;但种群杂合子缺失较严重,显著偏离Hardy-Weinberg平衡。(2)亚种群间Fst在0.088~0.181范围内,整体Fst=0.130,竹节树种群遗传分化较为显著。(3)竹节树种群有较大的花粉流及种子流,分别为131.78 m(50.13~186.36 m)和289.64 m。(4)竹节树种群空间遗传结构系数Sp为0.041,在4.4~7.0 m内有显著空间遗传结构。说明该种群尚处于健康和稳定的状态。

Based on SSR sites,we study the genetic structure,breeding system,gene flow and Finescale Spatial Genetic Structure of a natural Carallia brachiata population.The results show that:(1)The genetic diversity of the population is at a high level,Na=9.285,He=0.607 and I=1.437.The lack of heterozygosity is serious,and it deviates significantly from the Hardy-Weinberg equilibrium.(2)Fst between each subpopulation ranges from 0.088 to 0.181 with a total Fst of 0.130 in this population.In general,the population has a large genetic variation.(3)The pollen flow(131.78 m)and seed flow(289.64 m)of the population are large.(4)The genetic structure coefficient Sp of the population is 0.041,and there is a significant spatial genetic structure in the range of 4.4 m and 7.0 m.