Syndecan-1与表皮生长因子受体在甲状腺微小乳头状癌组织中的表达水平及其与临床病理特征的相关性分析

Expression level of Syndecan-1 and EGFR in micro papillary carcinoma tissue of thyroid and the correlation analysis between it and clinicopathologic features

目的:分析表皮生长因子受体(EGFR)和跨膜硫酸乙酰肝泰蛋白多糖(HSPGs)家族成员syndecan-1在甲状腺微小乳头状癌组织中的表达水平及其与临床病理特征的相关性。方法:选取医院收治的95例甲状腺微小乳头状癌患者的临床资料,将收集的肿瘤组织切除标本纳入恶性肿瘤组(95份),将癌旁正常组织标本纳入正常对照组(95份),另收集45例甲状腺良性肿瘤患者的临床资料,将其腺瘤组织标本纳入良性肿瘤组(45份)。通过免疫组织化学EnVision法对各组标本中EGFR和syndecan-1表达情况进行检测,分析EGFR、syndecan-1表达水平与甲状腺微小乳头状癌患者临床病理特征的相关性。结果:恶性肿瘤组EGFR阳性表达率较正常对照组和良性肿瘤组均明显升高,其差异有统计学意义(x^2=91.95,P<0.05);良性肿瘤组EGFR阳性率较正常对照组明显升高,其差异有统计学意义(x^2=95.31,P<0.05);恶性肿瘤组与正常对照组syndecan-1阳性表达率的比较,差异无统计学意义;恶性肿瘤组syndecan-1阳性表达率较良性肿瘤组明显下降,其差异有统计学意义(x^2=6.68,P<0.05);不同性别、年龄和是否伴有淋巴结转移的甲状腺微小乳头状癌患者EGFR阳性表达率的比较,差异有统计学意义(x^2=6.55,x^2=5.12,x^2=4.60;P<0.05);不同性别、年龄和是否伴有淋巴结转移者syndecan-1表达情况的比较,差异无统计学意义。结论:EGFR和syndecan-1均异常表达于甲状腺微小乳头状癌组织,二者在肿瘤侵袭和转移过程中可能起到重要的促进作用。

Objective: To analyze the expression level of epidermal growth factor receptor(EGFR) and syndecan-1 of heparan sulfate proteoglycan(HSPGs) in micro papillary carcinoma tissue of thyroid and the correlation between the level and clinicopathologic features. Methods: The clinical data of 95 patients with micro papillary carcinoma of thyroid in our hospital were selected. The collected excision specimens of tumor tissue were divided into malignant tumor group(95 cases) and adjacently normal tissue specimens were divided into normal control group(95 cases). And the clinical data of the specimens of 45 patients with benign tumor of thyroid were collected and their specimens were divided into benign tumor group. And the EnVision method of immunohistochemistry was used to detect the expression levels of EGFR and syndecan-1 in each group. And then, the correlation between the expression levels of EGFR and syndecan-1 and the clinicopathological characteristics of patients with micro papillary carcinoma was further analyzed. Results: The positive rate of EGFR expression in malignant tumors group was significantly higher than that in normal control group and benign tumors group(x^2=91.95, P<0.05), respectively. And the positive rate of EGFR in benign tumors group was significantly higher than that in normal control group(x^2=95.31, P<0.05). There was no significant difference in syndecan-1 positive expression rate between malignant tumor group and normal control group, and that of malignant tumor group was significantly lower than that of benign tumor group(x^2=6.68, P<0.05). And the differences of EGFR positive rates of patients with micro papillary carcinoma of thyroid among different genders, different ages and whether with or without lymphatic metastasis were significant(x^2=6.55, x^2=5.12, x^2=4.60, P<0.05). There were no significant difference in syndecan-1 expression among different genders, different ages and whether with or without lymphatic metastasis in patients with micro papillary carcinoma of thyroid. Conclusion: Both EGFR and syndecan-1 are abnormally expressed in micro papillary carcinoma tissue of thyroid, and they may play an important role in the process of invasion and metastasis of tumor.