中药复方脊髓康促进脊髓损伤大鼠神经功能恢复的实验研究

Experimental study on Chinese medicine compound Jisuikang promoting the recovery of neurological function in rats with spinal cord injury

目的验证中药复方脊髓康对脊髓损伤大鼠神经功能的作用,尝试探讨其对星形胶质细胞神经胶质原纤维酸性蛋白(Glial fibrillary acidicprotein,GFAP)表达的影响。方法采用改良Allen法建立大鼠脊髓损伤动物模型,模型成功建立后,将大鼠分为6组:假手术组、模型组、强的松组、脊髓康高剂量组、脊髓康中剂量组和脊髓康低剂量组。各组大鼠干预后3天、7天、14天各随机行斜板实验、肌力检测评价;观测每组分别在3天、7天、14天时的脊髓损伤区脊髓组织GFAP的变化并分析各组GFAP mRNA的情况。结果大鼠造模后予中药复方脊髓康灌胃,大鼠的斜板实验和双后肢神经功能评分明显优于模型组,表明了中药复方脊髓康能促进大鼠的神经功能恢复。免疫组化、荧光定量PCR显示,模型组GFAP mRNA表达量在各个时间点均明显高于强的松组及脊髓康高、中、低剂量组(P<0.05)。强的松组、中药复方脊髓康高、中、低剂量组在3天、7天、14天各时间点GFAP mRNA均高于模型组(P<0.05),脊髓损伤后3天时至最高峰,之后逐渐下降。结论研究明确了中药复方脊髓康对脊髓损伤大鼠星形胶质细胞GFAP表达的影响及对脊髓损伤后神经修复的价值。

Objective To verify Chinese medicine Jisuikang promoting the recovery of neurological function in rats with spinal cord injury,and to explore its effect on GFAP’s expression in astrocytes.Methods The rat model with spinal cord injury was established by modified Allen method.After modeling successfully,the rats were divided into 6 groups,including sham operation group,model group,prednisone group,high,medium and low dose groups.3,7 and 14 days after intervention,the inclined plate test and muscle strength detection and evaluation test were carried out randomly in each group.The changes of spinal cord tissue’s GFAP in spinal cord injury area at 3,7 and 14 days in each group were observed,and the changes of GFAP mRNA in each group was analyzed.Results After modeling,rats were given a gavage of Chinese medicine Jisuikang.The scores of inclined plane test and nerve function of bilateral hind limb in three Chinese compound groups all were significantly better than that in model group,indicating that Jisuikang can promote the recovery of nerve function.The results of immunohistochemistry and fluorescence quantitative PCR showed that,the expression levels of GFAP mRNA at each time point in model group,were significantly lower than those in prednisone group,high,medium and low dose of Jisuikang groups(P<0.05).Compared with the model grup,the expression levels of GFAP mRNA in prednisone group,high,medium and low dose of Jisuikang groups were decreased at each point time on 3 days,7 days and 14 days.It reached the peak at 3 days after spinal cord injury and then decreased gradually.Conclusion The study clarified the effect of Chinese medicine Jisuikang on the GFAP expression of astrocytes in rats with spinal cord injury and its value in nerve repair after spinal cord injury.