维生素E促进小鼠H-22移植瘤的作用及机制

Promoting effect of vitamin E on H-22 transplanted tumor in mice

[目的]利用H-22荷瘤小鼠研究维生素E(vitamin E,VE)对肿瘤发生发展的作用及机制。[方法]建立H-22小鼠荷瘤模型,计算肿瘤指数与肝脏指数,利用试剂盒检测血糖、血清低密度脂蛋白(low density lipoprotein,LDL)、总胆固醇(total cholesterol,TC)以及碱性磷酸酶(alkaline phosphatase,AKP)水平,利用苏木素-伊红染色与Real-time PCR检测VE对肿瘤发展和转移的作用及分子机制。[结果]肿瘤-VE组LDL水平、肿瘤组和肿瘤-VE组TC、AKP水平显著高于对照组(LDL,P <0.05;TC,P <0.01;AKP,P <0.001)。肿瘤组织中肿瘤-VE组小鼠p27K ip1、Stat3以及Cxcl12基因表达水平显著高于肿瘤组(p27Kip1和Stat3,P <0.05,Cxcl12,P <0.01)。肝脏组织中肿瘤-VE组Jak2、Pigf及Cxcl12基因表达水平显著高于肿瘤组(P <0.05),且肿瘤组Jak2、Pigf及Cxcl12基因表达水平显著高于对照组(P <0.05)。[结论] VE处理的H-22荷瘤小鼠肿瘤组织中p27Kip1、Stat3及Cxcl12表达水平显著升高,肝脏组织中Jak2、Pigf及Cxcl12表达水平显著升高,VE可能通过调控细胞周期、肿瘤转移及炎症相关基因的表达促进小鼠H-22肿瘤的发展。

[Objective] The effects and underlying mechanisms of vitamin E(VE) in the development and metastasis of tumor were studied in H-22 tumor-bearing mice.[Method] H-22 tumor-bearing mice model was established. Tumor index and liver index were calculated,and blood glucose,serum low density lipoprotein(LDL),total cholesterol(TC) and alkaline phosphatase(AKP) levels were checked using detection kits. Hematoxylin-eosin staining and Real-time PCR were used to study the effects of VE on the development and metastasis of tumor using the H-22 tumor-bearing mice.[Result]The LDL level of the tumor-VE group,the TC and AKP levels of the tumor-VE group and the tumor group were significantly increased compared with the control group(LDL,P < 0. 05;TC,P < 0. 01;AKP,P < 0. 001). The expression of p27 Kip1,Stat3 and Cxcl12 in tumor tissue of the tumor-VE group was significantly upregulated compared with the tumor group(p27 Kip1 and Stat3,P < 0. 05;Cxcl12,P < 0. 01). The expression level of Jak2,Pigf and Cxcl12 in liver tissue of the tumor-VE group was significantly upregulated compared with the tumor group(P < 0. 05),and the expression level of Jak2,Pigf and Cxcl12 in liver tissue of the tumor group was significantly upregulated compared with the control group(P < 0. 05).[Conclusion]The expression of p27 Kip1,Stat3 and Cxcl12 in the tumor tissue of the H-22 bearing mice treated with VE was significantly increased. The expression of Jak2,Pigf and Cxcl12 in the liver tissue of the H-22 bearing mice treated with VE was significantly increased. VE might promote the development and metastasis of tumor by regulating the expression of genes related with cell cycle,inflammation and metastasis.