玉屏风颗粒对变应性鼻炎大鼠Th17/Treg平衡及相关细胞因子的影响

Effects of Yupingfeng Formula Granules on Th17/Treg balance and related cytokines of rats with allergic rhinitis

目的研究玉屏风颗粒对变应性大鼠Th17/Treg免疫平衡的影响。方法采用OVA制备AR大鼠模型,大鼠随机分为正常组、模型组、氯雷他定组、玉屏风颗粒组(12、6 g/kg)。各组干预后评估大鼠AR行为学总分,HE和AB-PAS染色观察鼻黏膜组织病变情况,ELISA检测AR大鼠血清中的IL-17、TGF-β1水平,RT-PCR检测鼻黏膜组织中RORγt mRNA、Foxp3 mRNA的表达,Western blot检测鼻黏膜组织中RORγt、Foxp3蛋白表达。结果与模型组比较,玉屏风颗粒组(12、6 g/kg)大鼠挠鼻次数、打喷嚏次数等行为学总分降低(P<0.05);上皮细胞结构完整、少量炎症细胞浸润、杯状细胞化生明显减少;血清中的IL-17水平降低、TGF-β1水平升高(P<0.05);鼻黏膜RORγt mRNA表达降低、Foxp3 mRNA表达升高(P<0.05);鼻黏膜组织RORγt/Foxp3蛋白表达比例升高(P<0.05)。结论玉屏风颗粒能明显改善变应性大鼠鼻黏膜炎性症状,其作用机制可能与调节Th17/Treg平衡及相关细胞因子有关。

AIM To study the effects of Yupingfeng Formula Granules(YFG)on Th17/Treg immune balance in rats with allergic rhinitis(AR).METHODS The AR rat model was established by sensitization and stimulation with ovalbumin.Forty SD rats were randomly divided into normal group,model group,loratadine group,YFG groups(dosed at 12 g/kg or 6 g/kg).The rats were subjected to the assessment of the total score of AR behavior,the observation of the pathological changes of nasal mucosa by HE and AB-PAS staining,the detection of serum level of IL-17,TGF-β1 by ELISA,the detection of the expression of RORγt mRNA and Foxp3 mRNA in nasal mucosa by RT-PCR,the detection of the protein expression of RORγt and Foxp3 by Western blot.RESULTS In contrast to the model group,YFG groups shared lower scores in terms of rubbing,sneezing and other behaviors(P<0.05);most intact epithelial cells,less amount of infiltrated inflammatory cells,significantly decreased goblet cell metaplasia;decreased IL-17 level and increased TGF-β1 level in serum(P<0.05);decreased RORγt mRNA expression and increased Foxp3 mRNA expression in nasal mucosa(P<0.05);and increased ratio of RORγt/Foxp3 protein expression(P<0.05).CONCLUSION YFG can significantly improve the inflammatory symptoms of nasal mucosa in AR rats,and its mechanism may be associated with the regulation of Th17/Treg balance and the relevant cytokines.