UPLC同时测定蜘蛛香中9种成分

Simultaneous determination of nine constituents in Valeriana Jatamansi by UPLC

目的建立UPLC法同时测定蜘蛛香Valeriana jatamansi Jones中新绿原酸、绿原酸、咖啡酸、橙皮苷、异绿原酸B、异绿原酸A、异绿原酸C、乙酰缬草三酯、缬草三酯的含有量。方法蜘蛛香70%甲醇提取液的分析采用Ultimate^■UHPLC Polar-RP色谱柱(2.1 mm×100 mm,1.8μm);流动相乙腈-0.1%甲酸水溶液,梯度洗脱;检测波长327 nm(0~11 min)、256 nm(11~30 min);体积流量0.21 mL/min;柱温30℃。结果9种成分在各自范围内线性关系良好(r≥0.9999),平均加样回收率103.54%~108.28%,RSD 0.70%~2.20%。结论该方法准确稳定,重复性好,可用于蜘蛛香的质量控制。

AIM To establish a UPLC method for simultaneous determination of neochlorogenic acid,chlorogenic acid,caffeic acid,hesperidin,isochlorogenic acid B,isochlorogenic acid A,isochlorogenic acid C,acevaltrate and valepotriate in Valeriana jatamansi Jones.METHODS The analysis of 70%ethanol extract of V.Jatamansi was performed on a 30℃thermostatic Ultimate^■UHPLC Polar-RP(2.1 mm×100 mm,1.8μm),with the mobile phase comprising of acetonitrile-0.1%formic acid flowing at 0.21 mL/min in a gradient elution manner,and the detection wavalengths were set at 327 nm(0-11 min)and 256 nm(11-30 min).RESULTS Nine constituents showed good linear relationships within their own ranges(r≥0.9999),whose average recoveries were 103.54%-108.28%with the RSDs of 0.70%-2.20%.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of V.jatamansi.