ELISA法快速检测中药材黄曲霉毒素总量

Rapid detection of total aflatoxin in traditional herbal medicines by ELISA

目的建立ELISA法快速检测中药材黄曲霉毒素总量。方法建立间接竞争ELISA法并对该方法进行封闭液、包被液、包被方式、竞争反应时间、显色时间、离子强度优化;对6种中药材前处理优化,检测6种中药材黄曲霉毒素总量;并采用HPLC法测定6种中药材中黄曲霉毒素总量,并对ELISA法进行验证。结果优化后黄曲霉毒素总量在6.25~10 ng/L范围内线性关系良好(r=0.9902)。6种中药材平均回收率94.5%~109.0%,RSD 5.11%~14.56%。ELISA检测结果与HPLC法一致。结论该方法操作简单、快速,可用于快速检测黄曲霉毒素总量。

AIM To establish a rapid ELISA method for the determination of total aflatoxin in traditional herbal medicines.METHODS An indirect competitive ELISA method was established,which optimized blocking solution,coating solution,coating method,competitive reaction time,coloration time and ionic strength.The pretreatment of six traditional herbal medicines were optimized,and the determination of total aflatoxin was detected by ELISA method.The aflatoxins total of six traditional herbal medicines was detected by HPLC method to verify ELISA method.RESULTS Total aflatoxin showed good linear relationships within 6.25-10 ng/L(r=0.9902).The average recoveries of six traditional herbal medicines were 94.5%-109.0%with the RSDs of 5.11%-14.56%.The results of ELISA were consistent with the results of HPLC.CONCLUSION This simple and rapid method can be used for the determination of total aflatoxin in traditional herbal medicines.